MEK2 Antibody - middle region (OAAB17346)

Catalog No: OAAB17346

Size:400ul

The anti-MAP2K2 Pab (Cat. #OAAB17346) is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.

Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center) (Cat.#OAAB17346).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies).

MAP2K2 Antibody (A278) (Cat. #OAAB17346) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody (Center)(Cat#OAAB17346/SA110819AW).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MEK2 immunoreactivity is localized to Cytoplasm significantly.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: MEK2 (MAP2K2) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the Central region of human MEK2 (MAP2K2).
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that MAP2K2 is expressed in HEK293

• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:50~100

Target Info

• Gene Symbol: MAP2K2
• Alias Symbols: CFC4, MEK2, MKK2, MAPKK2, PRKMK2
• NCBI Gene Id: 5605
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P36507
• Protein Accession #: NP_109587.1
• Molecular Weight: 44424 Da