FGR Antibody - N-terminal region (OAAB17222)

• Product Number: OAAB17222
• Name: FGR Antibody - N-terminal region (OAAB17222)
• Molecular Weight: 59479 Da
• Isotype: Ig
• NCBI Gene Id: 2268
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: SRC2, c-fgr, c-src2, p55-Fgr, p58-Fgr, p55c-fgr, p58c-fgr
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: FGR Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK- dependent activation of AKT1 and MAP kinase signaling Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1
• Application Info: WB: 1:2000
  WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
• Immunogen: This FGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-33 amino acids from the N-terminal region of human FGR.
• Additional Information: Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side (Probable). Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Mitochondrion inner membrane (By similarity). Mitochondrion intermembrane space (By similarity) Note=Detected in mitochondrial intermembrane space and at inner membranes (By similarity). Colocalizes wi
  Tissue Location: Detected in neutrophils, monocytes and natural killer cells (at protein level). Detected in monocytes and large lymphocytes
• Uniprot ID: P09769
• Protein Name: Tyrosine-protein kinase Fgr
• Protein Accession #: NP_001036194.1
• Gene Symbol: FGR
• Predicted Species Reactivity: Human
• Application: WB, IHC, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: 293 cell
  
  Western blot analysis of FGR (arrow) using FGR Antibody (N-term) (Cat.#OAAB17222). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGR gene (Lane 2) (Origene Technologies).
• Image 2: Ramos cell
  
  The anti-FGR Pab (Cat. #OAAB17222) is used in Western blot to detect FGR in Ramos cell lysate.
• Image 3: human cancer tissue
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 4: MDA-MB231 cell
  
  Confocal immunofluorescent analysis of FGR Antibody (N-term)(Cat#OAAB17222) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).