ABL1 Antibody - N-terminal region (OAAB17212)

• Product Number: OAAB17212
• Name: ABL1 Antibody - N-terminal region (OAAB17212)
• Molecular Weight: 123 kDa
• Isotype: IgG
• NCBI Gene Id: 25
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.28 mg/ml
• Alias Symbols: ABL, JTK7, p150, c-ABL, v-abl, CHDSKM, c-ABL1, BCR-ABL, bcr/abl
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Reference: Donaldson, L.W., et al., Proc. Natl. Acad. Sci. U.S.A. 99(22):14053-14058 (2002).
  Pisabarro, M.T., et al., J. Mol. Biol. 281(3):513-521 (1998).
  Nam, H.J., et al., Structure 4(9):1105-1114 (1996).
  Chissoe, S.L., et al., Genomics 27(1):67-82 (1995).
  Gosser, Y.Q., et al., Structure 3(10):1075-1086 (1995).
• Description of Target: The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired Phosphorylates TP73, a primary regulator for this type of damage- induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin- associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21- dependent manner. Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity.
• Additional Information: Cellular Location: Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Note=Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress.
  Tissue Location: Widely expressed.
• Application Info: WB: 1:1,000
  Optimal dilutions should be determined by the end user.
• Immunogen: This ABL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the N-terminal region of human ABL1.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P00519
• Protein Name: Tyrosine-protein kinase ABL1
• Protein Accession #: NP_005148.2
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Tested Species Reactivity: Human
• Gene Symbol: ABL1
• Predicted Species Reactivity: Mouse, Zebrafish
• Application: WB
• Image 1: Y79
  
  Western blot analysis of ABL1 Antibody (N-term H246) (Cat.# OAAB17212) in Y79 cell line lysates (35ug/lane). ABL1(arrow) was detected using the purified Pab (1:60 dilution).