TSC1 Antibody - middle region (OAAB16678)

• Product Number: OAAB16678
• Name: TSC1 Antibody - middle region (OAAB16678)
• Molecular Weight: 129767 Da
• Isotype: Ig
• NCBI Gene Id: 7248
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: LAM, TSC
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: Hamartin (TSC1) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50
• Immunogen: This Hamartin (TSC1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the Central region of human Hamartin (TSC1).
• Additional Information: Cellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes
  Tissue Location: Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells
• Uniprot ID: Q92574
• Protein Name: Hamartin
• Protein Accession #: NP_000359.1
• Gene Symbol: TSC1
• Predicted Species Reactivity: Human
• Application: WB, IHC, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Human Skeletal Muscle
  
  Hamartin (TSC1) Antibody (Center) (Cat. # OAAB16678) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hamartin (TSC1) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: Hela, Nuclear
  
  Confocal immunofluorescent analysis of Hamartin (TSC1) Antibody (Center) (Cat# OAAB16678) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
• Image 3: MDA-MB231
  
  TSC1 Antibody (Center) (Cat. #OAAB16678) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TSC1 antibody detected the TSC1 protein (arrow).
• Image 4: Human skeletal muscle
  
  Hamartin (TSC1) Antibody (Center) (Cat. #OAAB16678)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hamartin (TSC1) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 5: Hela
  
  Confocal immunofluorescent analysis of Hamartin (TSC1) Antibody (Center)(Cat#OAAB16678) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).