MMP16 Antibody - N-terminal region (OAAB16542)

• Product Number: OAAB16542
• Name: MMP16 Antibody - N-terminal region (OAAB16542)
• Molecular Weight: 69521 Da
• Isotype: Ig
• NCBI Gene Id: 4325
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: MMP-X2, C8orf57, MT-MMP2, MT-MMP3, MT3-MMP
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MMP16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells
• Application Info: IHC-P: 1:50~100
  WB: 1:1000
• Immunogen: This MMP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human MMP16.
• Additional Information: Cellular Location: Isoform Long: Cell membrane; Single-pass type I membrane protein; Extracellular side (Potential) Note=Localized at the cell surface of melanoma cells
  Tissue Location: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary
• Uniprot ID: P51512
• Protein Name: Matrix metalloproteinase-16
• Sample Type Confirmation:

  MMP16 is supported by BioGPS gene expression data to be expressed in MDA-MB435

• Protein Accession #: NP_005932.2
• Gene Symbol: MMP16
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: MB435, MDA-MB435
  
  MMP16 Antibody (E169) (Cat. # OAAB16542) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MMP16 antibody detected the MMP16 protein (arrow).

  MMP16 is supported by BioGPS gene expression data to be expressed in MDA-MB435