MMP16 Antibody - N-terminal region (OAAB16542)

Catalog No: OAAB16542

Size:400ul

MMP16 Antibody (E169) (Cat. # OAAB16542) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MMP16 antibody detected the MMP16 protein (arrow).

MMP16 is supported by BioGPS gene expression data to be expressed in MDA-MB435

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC, WB
• Additional Information: Precautions: MMP16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells
  Cellular Location: Isoform Long: Cell membrane; Single-pass type I membrane protein; Extracellular side (Potential) Note=Localized at the cell surface of melanoma cells
  Tissue Location: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MMP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human MMP16.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  MMP16 is supported by BioGPS gene expression data to be expressed in MDA-MB435

• Application Info: IHC-P: 1:50~100
  WB: 1:1000

Target Info

• Gene Symbol: MMP16
• Alias Symbols: MMP-X2, C8orf57, MT-MMP2, MT-MMP3, MT3-MMP
• NCBI Gene Id: 4325
• Protein Name: Matrix metalloproteinase-16
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P51512
• Protein Accession #: NP_005932.2
• Molecular Weight: 69521 Da