CXCL12 Antibody - C-terminal region (OAAB09710)

• Product Number: OAAB09710
• Name: CXCL12 Antibody - C-terminal region (OAAB09710)
• Molecular Weight: 11 kDa
• Isotype: Ig
• NCBI Gene Id: 6387
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml. Actual concentration varies with each lot.
• Alias Symbols: IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  Cellular Location: Secreted.
  Tissue Location: Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This CXCL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the C-terminal region of human CXCL12.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P48061
• Protein Name: Stromal cell-derived factor 1
• Publications:

  de Paz, J. L. et al. Profiling heparin-chemokine interactions using synthetic tools. ACS Chem. Biol. 2, 735-44 (2007). 18030990

• Protein Accession #: NP_000600.1
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: CXCL12
• Predicted Species Reactivity: Human
• Application: WB, IHC-P
• Image 1: NCI-H292
  
  CXCL12 Antibody (C-term) (Cat. #OAAB09710) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CXCL12 antibody detected the CXCL12 protein (arrow).
• Image 2: Human Liver
  
  CXCL12 Antibody (C-term) (Cat. #OAAB09710)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CXCL12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.