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CXCL12 Antibody - C-terminal region (OAAB09710)

400ul
$320.00
In Stock
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Gene Symbol:
CXCL12
NCBI Gene Id:
6387
Protein Name:
Stromal cell-derived factor 1
Swissprot Id:
P48061
Protein Accession #:
NP_000600.1
Alias Symbols:
Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, SDF-1-beta(3-72), SDF-1-alpha(3-67), CXCL12, SDF1, SDF1A, SDF1B
Replacement Item:
This antibody may replace item sc-133989 from Santa Cruz Biotechnology.
Description of Target:
This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
Molecular Weight:
11 kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application:
WB, IHC-P
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CXCL12.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CXCL12.
Immunogen:
This CXCL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the C-terminal region of human CXCL12.
Product Format:
Liquid. PBS with 0.09% (W/V) sodium azide.
Reconstitution and Storage:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Concentration:
0.5 mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals:
Printable datasheet for OAAB09710
Isotype:
Ig
Intended Use:
For research use only and not for use in diagnostic or therapeutic procedures.
Application Info:
WB 1:1000
IHC-P 1:10~50
:::
Function: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Cellular Location: Secreted.
Tissue Location: Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
Publications:

de Paz, J. L. et al. Profiling heparin-chemokine interactions using synthetic tools. ACS Chem. Biol. 2, 735-44 (2007). WB, IHC, Human 18030990

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