KCTD10 Antibody - C-terminal region (OAAB09706)

• Product Number: OAAB09706
• Name: KCTD10 Antibody - C-terminal region (OAAB09706)
• Molecular Weight: 35432 Da
• Isotype: Ig
• NCBI Gene Id: 83892
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: BTBD28, ULRO61, MSTP028, hBACURD3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: KCTD10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. The BCR(BACURD3) E3 ubiquitin ligase complex mediates the ubiquitination of target proteins, leading to their degradation by the proteasome (By similarity)
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Immunogen: This KCTD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human KCTD10.
• Additional Information: Cellular Location: Nucleus.
• Uniprot ID: Q9H3F6
• Protein Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3
• Publications:

  Wang, Y. et al. KCTD10 interacts with proliferating cell nuclear antigen and its down-regulation could inhibit cell proliferation. J. Cell. Biochem. 106, 409-13 (2009). 19125419

• Protein Accession #: NP_114160.1
• Gene Symbol: KCTD10
• Predicted Species Reactivity: Human
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Jurkat
  
  KCTD10 Antibody (C-term) (Cat. #OAAB09706) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
• Image 2: Jurkat
  
  KCTD10 Antibody (C-term) (Cat. #OAAB09706) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the KCTD10 antibody detected the KCTD10 protein (arrow).
• Image 3: Human Brain
  
  KCTD10 Antibody (C-term) (Cat. #OAAB09706)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCTD10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.