MED25 Antibody - N-terminal region (OAAB00209)

• Product Number: OAAB00209
• Name: MED25 Antibody - N-terminal region (OAAB00209)
• Molecular Weight: 78 kDa
• Isotype: IgG
• NCBI Gene Id: 81857
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.39 mg/mL
• Alias Symbols: P78, ACID1, ARC92, BVSYS, PTOV2, CMT2B2, TCBAP0758
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Nucleus
  Tissue Location: Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids of human MED25.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q71SY5
• Protein Name: Mediator of RNA polymerase II transcription subunit 25
• Publications:

  Synergistic activation of Arg1 gene by retinoic acid and IL-4 involves chromatin remodeling for transcription initiation and elongation coupling. Nucleic Acids Res. 44, 7568-79 (2016). 27166374

• Protein Accession #: NP_112235.2
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: MED25
• Predicted Species Reactivity: Human, Mouse, Bovine
• Application: WB, IHC-P
• Image 1: human brain
  
  MED25 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: MCF-7
  
  MED25 Antibody (N-term) ( western blot analysis in MCF-7 cell lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).
• Image 3: Mouse Kidney
  
  MED25 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).