ROBO3 Antibody - C-terminal region (ARP74184_P050)

• Product Number: ARP74184_P050
• Name: ROBO3 Antibody - C-terminal region (ARP74184_P050)
• Protein Size (# AA): 1386 amino acids
• Molecular Weight: 152kDa
• NCBI Gene Id: 64221
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Alias Symbols: HGPS, RIG1, HGPPS, RBIG1, HGPPS1
• Peptide Sequence: Synthetic peptide located within the following region: HPSRSPSAQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPGEGP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated.
• Protein Interactions: CCDC57; LNX1; RBMX; MTUS2; Zswim8; APOA1; NEDD4L; UBC;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ROBO3 (ARP74184_P050) antibody is Catalog # AAP74184
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human ROBO3
• Uniprot ID: Q96MS0
• Protein Accession #: NP_071765
• Purification: Affinity Purified
• Tested Species Reactivity: Human
• Gene Symbol: ROBO3
• Predicted Species Reactivity: Human
• Application: WB
• Image 1: Human 721_B Whole Cell
  
  Host: Rabbit
  Target Name: ROBO3
  Sample Type: 721_B Whole Cell lysates
  Antibody Dilution: 1ug/ml
• Image 2: Human Jurkat Whole Cell
  
  Host: Rabbit
  Target Name: ROBO3
  Sample Type: Jurkat Whole Cell lysates
  Antibody Dilution: 1.0ug/ml