| Product Number |
ARP52242_P050 |
| Product Page |
www.avivasysbio.com/cops8-antibody-middle-region-arp52242-p050.html |
| Name |
COPS8 Antibody - middle region (ARP52242_P050) |
| Protein Size (# AA) |
209 amino acids |
| Molecular Weight |
23kDa |
| Subunit |
8 |
| NCBI Gene Id |
10920 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis) |
| Alias Symbols |
COP9, CSN8, SGN8 |
| Peptide Sequence |
Synthetic peptide located within the following region: TRMVLPRKPVAGALDVSFNKFIPLSEPAPVPPIPNEQQLARLTDYVAFLE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Description of Target |
The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Protein Interactions |
USHBP1; GPS1; cul1; WDR77; SRRT; COPS7A; VIM; COPS4; FBXO6; FBXW4; COPS5; vpr; IRF5; DDIT3; COPS2; COPS3; COPS6; PINK1; RFWD2; CUL4A; UBC; LRR1; DDA1; DDB2; DCUN1D1; VPRBP; CUL2; CUL3; CUL4B; CUL5; NEDD8; JUN; GFER; ERCC8; DDB1; CTNNB1; RAE1; COPS8; EIF3E |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-COPS8 (ARP52242_P050) antibody |
| Blocking Peptide |
For anti-COPS8 (ARP52242_P050) antibody is Catalog # AAP52242 (Previous Catalog # AAPP44002) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human COPS8 |
| Uniprot ID |
Q99627 |
| Protein Name |
COP9 signalosome complex subunit 8 |
| Publications |
Pick, E. et al. The minimal deneddylase core of the COP9 signalosome excludes the Csn6 MPN- domain. PLoS One 7, e43980 (2012). 22956996 |
| Sample Type Confirmation |
There is BioGPS gene expression data showing that COPS8 is expressed in HepG2 |
| Protein Accession # |
NP_006701 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_006710 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
COPS8 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
| Image 1 | Human HepG2
 | WB Suggested Anti-COPS8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that COPS8 is expressed in HepG2 |
|
| Image 2 | Human, Mouse
 | Sample Type: 1. B8 mouse fibroblast cells, extraction only
2. HEK 293 cells, extraction only
3. HEK 293 cells, purified
4. Human Blood cells, partially pure
5. Human Blood cells, pure
6. S. pombe, extraction only
Primary Dilution: 1:200
Secondary Anitbody: HRP conjugated anti-rabbit
Image Submitted By: Dr. Elah Pick
University of Haifa at Oranim |
|
| Image 3 | Human liver tissue
 | Rabbit Anti-COPS8 Antibody
Catalog Number: ARP52242_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm and nucleus in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
