CDYL Antibody - N-terminal region (ARP49096_P050)

• Product Number: ARP49096_P050
• Name: CDYL Antibody - N-terminal region (ARP49096_P050)
• Protein Size (# AA): 544 amino acids
• Molecular Weight: 60kDa
• NCBI Gene Id: 9425
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Chromodomain protein, Y-like
• Alias Symbols: CDYL1
• Peptide Sequence: Synthetic peptide located within the following region: YIHDFNRRHTEKQKESTLTRTNRTSPNNARKQISRSTNSNFSKTSPKALV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Nousiainen,M., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (14), 5391-5396
• Description of Target: CDYL acts as repressor of transcription. CDYL has histone acetyltransferase activity, with a preference for histone H4.Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.
• Protein Interactions: UBC; HECW2; HDAC2; HDAC1; MIER1; MIER2; EHMT2; RBBP4; MYC; SUZ12; EZH2; CTBP1; WIZ; ZNF644; SETDB1; SMARCB1; SMARCA4; REST; HIST1H3A; HTRA1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-CDYL (ARP49096_P050) antibody is Catalog # AAP49096 (Previous Catalog # AAPY02282)
• Immunogen: The immunogen is a synthetic peptide directed towards the n terminal region of human CDYL
• Uniprot ID: Q9Y232
• Protein Name: Chromodomain Y-like protein
• Publications:

  Li, J. et al. A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells. FEBS Lett. 584, 3198-202 (2010). 20547158

• Protein Accession #: NP_004815
• Purification: Affinity Purified
• Nucleotide Accession #: NM_004824
• Tested Species Reactivity: Human
• Gene Symbol: CDYL
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IF, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 79%; Rabbit: 93%; Rat: 86%
• Image 1: Transfected 293T
  
  WB Suggested Anti-CDYL Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: Transfected 293T
• Image 2: HeLa
  
  Sample Type:
  HeLa
  Primary Antibody Dilution:
  4 ug/ml
  Secondary Antibody:
  Anti-rabbit Alexa 546
  Secondary Antibody Dilution:
  2 ug/ml
  Gene Name:
  CDYL
• Image 3: MD MB231
  
  Sample Type:
  MD MB231
  Primary Antibody Dilution:
  4 ug/ml
  Secondary Antibody:
  Anti-rabbit Alexa 546
  Secondary Antibody Dilution:
  2 ug/ml
  Gene Name:
  CDYL