FBXW7 Antibody - C-terminal region (ARP47419_P050)

• Product Number: ARP47419_P050
• Name: FBXW7 Antibody - C-terminal region (ARP47419_P050)
• Protein Size (# AA): 707 amino acids
• Molecular Weight: 80 kDa
• NCBI Gene Id: 55294
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
• Description:
  View product citations for FBXW7 Antibody - C-terminal region (ARP47419_P050) on CiteAb
• Alias Symbols: AGO, CDC4, FBW6, FBW7, hAgo, FBX30, FBXW6, SEL10, hCdc4, FBXO30, SEL-10
• Peptide Sequence: Synthetic peptide located within the following region: LKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.
• Protein Interactions: MCL1; CDC34; CCNE1; FBXW7; CUL1; UBE2D1; PIN1; DAB2IP; YAP1; SKP1; MAP4K1; EYA1; KLF5; SREBF1; NOTCH1; UBE2QL1; Creb3l2; Creb3l1; SIC1; CSF3R; KLF2; NCOA3; PTEN; NFE2L1; HSP90AA1; MED11; MED19; MED8; MED30; MED10; MED28; MED29; MED9; MED15; MED31; MED4; M
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Additional Information: IHC Information: Paraffin embedded kidney tissue, tested with an antibody dilution of 2.5 ug/ml.
• Blocking Peptide: Catalog # AAP47419
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human FBXW7
• Uniprot ID: Q969H0-4
• Protein Name: F-box/WD repeat-containing protein 7
• Publications:

  FBXW7 mediates high glucose‑induced SREBP‑1 expression in renal tubular cells of diabetic nephropathy under PI3K/Akt pathway regulation. Mol Med Rep. 23 (2021). 33537812

  Gong, Y. et al. Pan-cancer genetic analysis identifies PARK2 as a master regulator of G1/S cyclins. Nat. Genet. 46, 588-94 (2014). 24793136

• Sample Type Confirmation:

  FBXW7 is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_001013433
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001013415
• Tested Species Reactivity: Human
• Gene Symbol: FBXW7
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Fetal Heart
  
  Host: Rabbit
  Target Name: GNAS
  Sample Type: Human Fetal Heart
  Antibody Dilution: 1.0ug/ml
• Image 2: Human Kidney
  
  Kidney
• Image 3: Human Pineal Tissue
  
  Rabbit Anti-FBXW7 Antibody
  Catalog Number: ARP47419_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
  Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1/600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 4: Human Jurkat Whole Cell
  
  Host: Rabbit
  Target Name: FBXW7
  Sample Tissue: Human Jurkat Whole Cell
  Antibody Dilution: 1ug/ml