HIST1H1C Antibody - middle region (ARP46122_P050)

• Product Number: ARP46122_P050
• Name: HIST1H1C Antibody - middle region (ARP46122_P050)
• Protein Size (# AA): 213 amino acids
• Molecular Weight: 21kDa
• NCBI Gene Id: 3006
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Histone cluster 1, H1c
• Alias Symbols: H1C, H1.2, H1F2, H1s-1, HIST1H1C
• Peptide Sequence: Synthetic peptide located within the following region: ASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kim,K., (2008) J. Biol. Chem. 283 (14), 9113-9126
• Description of Target: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: UBC; CEP250; ATRX; HIST1H3A; LEO1; PAF1; CUL4A; POLR2A; MDM2; ASB16; ASB9; SUZ12; EED; RNF2; APEH; SEC24D; TBCD; PARK2; VHL; UBD; ICAM1; CD81; IGSF8; EIF2AK2; ITGA4; IL7R; CSNK2A2; SMURF1; HIST1H2AD; H1FX; COPS5; CAND1; CUL1; CUL2; CUL3; CUL4B; CUL5; NEDD
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-HIST1H1C (ARP46122_P050) antibody is Catalog # AAP46122 (Previous Catalog # AAPP26984)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HIST1H1C
• Uniprot ID: P16403
• Protein Name: Histone H1.2
• Sample Type Confirmation:

  HIST1H1C is supported by BioGPS gene expression data to be expressed in 721_B

• Protein Accession #: NP_005310
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005319
• Tested Species Reactivity: Human
• Gene Symbol: HIST1H1C
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: CHIP, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 86%; Guinea Pig: 93%; Horse: 86%; Human: 100%; Mouse: 86%; Rabbit: 85%; Rat: 86%
• Image 1: Human 721_B
  
  WB Suggested Anti-HIST1H1C Antibody Titration: 0.2-1 ug/ml
  Positive Control: 721_B cell lysateHIST1H1C is supported by BioGPS gene expression data to be expressed in 721_B
• Image 2: HCT116
  
  Chromatin Immunoprecipitation (ChIP) Using HIST1H1C antibody - middle region (ARP46122_P050) and HCT116 Cells
• Image 3: Human Placenta
  
  Rabbit Anti-HIST1H1C antibody
  Catalog Number: ARP46122
  Formalin Fixed Paraffin Embedded Tissue: Human Placenta
  Primary antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20x
  Exposure Time: 0.5-2.0sec
• Image 4: Human Testis
  
  Rabbit Anti-HIST1H1C antibody
  Catalog Number: ARP46122
  Formalin Fixed Paraffin Embedded Tissue: Human Testis
  Primary antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20x
  Exposure Time: 0.5-2.0sec