GCDH Antibody - C-terminal region (ARP43559_T100)

• Product Number: ARP43559_T100
• Name: GCDH Antibody - C-terminal region (ARP43559_T100)
• Protein Size (# AA): 438 amino acids
• Molecular Weight: 48kDa
• NCBI Gene Id: 2639
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Glutaryl-CoA dehydrogenase
• Alias Symbols: GCD, ACAD5
• Peptide Sequence: Synthetic peptide located within the following region: IARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Description of Target: GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits.The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Protein Interactions: UBC; FBXO6; ADRB2; RNF32; EXOC6; ECSIT; MAST1; CDC37; PSEN2; PSEN1; NOS3; APOE; A2M; UBD; MYC; GCDH;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.
• Blocking Peptide: For anti-GCDH (ARP43559_T100) antibody is Catalog # AAP43559 (Previous Catalog # AAPP25004)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GCDH
• Uniprot ID: Q92947
• Protein Name: Glutaryl-CoA dehydrogenase, mitochondrial
• Publications:

  Hu, Z. et al. Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity. Theranostics 4, 215-28 (2014). 24465277

• Protein Accession #: NP_000150
• Purification: Protein A purified
• Nucleotide Accession #: NM_000159
• Tested Species Reactivity: Human
• Gene Symbol: GCDH
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Muscle
  
  Human Muscle
• Image 2: Human HepG2
  
  WB Suggested Antibody Titration: 2.5ug/ml
  Positive Control: HepG2