| Product Number |
ARP43283_P050 |
| Product Page |
www.avivasysbio.com/sh3rf1-antibody-middle-region-arp43283-p050.html |
| Name |
SH3RF1 Antibody - middle region (ARP43283_P050) |
| Protein Size (# AA) |
888 amino acids |
| Molecular Weight |
93kDa |
| NCBI Gene Id |
57630 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
SH3 domain containing ring finger 1 |
| Alias Symbols |
POSH, RNF142, SH3MD2 |
| Peptide Sequence |
Synthetic peptide located within the following region: LLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHG |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Lyons,T.R., (2007) J. Biol. Chem. 282 (30), 21987-21997 |
| Description of Target |
SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. |
| Protein Interactions |
YWHAB; UBE2N; UBE2D3; UBE2D1; AKT2; AKT1; SIAH2; SIAH1; SH3RF2; RAC1; SEMA6A; UBC; PDCD6IP; KCNJ1; MAP4K3; MAP3K9; ADAM15; PAK2; RACGAP1; MAP3K12; MAP3K10; MAP3K11; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SH3RF1 (ARP43283_P050) antibody |
| Blocking Peptide |
For anti-SH3RF1 (ARP43283_P050) antibody is Catalog # AAP43283 (Previous Catalog # AAPP11358) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human SH3RF1 |
| Uniprot ID |
Q7Z6J0 |
| Protein Name |
E3 ubiquitin-protein ligase SH3RF1 |
| Protein Accession # |
NP_065921 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_020870 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
SH3RF1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
| Image 1 | Human DU145
 | WB Suggested Anti-SH3RF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: DU145 cell lysate |
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