| Product Number |
ARP40716_T100 |
| Product Page |
www.avivasysbio.com/nono-antibody-c-terminal-region-arp40716-t100.html |
| Name |
NONO Antibody - C-terminal region (ARP40716_T100) |
| Protein Size (# AA) |
471 amino acids |
| Molecular Weight |
52kDa |
| NCBI Gene Id |
4841 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Non-POU domain containing, octamer-binding |
| Alias Symbols |
P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114 |
| Peptide Sequence |
Synthetic peptide located within the following region: DGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Kimura,K., (2006) Genome Res. 16 (1), 55-65 |
| Description of Target |
NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. |
| Protein Interactions |
SFPQ; PTBP1; PRKAA2; PIN1; NONO; FUS; DDX6; C11orf68; PSPC1; HUWE1; LMO4; UBC; SUMO2; STAU1; MDM2; RPA3; RPA2; RPA1; ASB18; WWOX; ASB3; ERG; EED; rev; WBP4; TCERG1; APBB1; PRPF40A; LMNA; CD81; PAN2; CTNNB1; ORC5; MYC; ITGA4; IL7R; FN1; EWSR1; YLPM1; RNF43 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NONO (ARP40716_T100) antibody |
| Blocking Peptide |
For anti-NONO (ARP40716_T100) antibody is Catalog # AAP40716 (Previous Catalog # AAPP10454) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human NONO |
| Uniprot ID |
Q15233 |
| Protein Name |
Non-POU domain-containing octamer-binding protein |
| Protein Accession # |
NP_031389 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_007363 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
NONO |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
IF, IHC, WB, IP |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100% |
| Image 1 | Human HepG2
 | WB Suggested Anti-NONO Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate |
|
| Image 2 | subnuclear bodies
 | Sample Type : subnuclear bodies-paraspeckles
|
|
| Image 3 | Human kidney
 | Human kidney |
|
| Image 4 | Human Liver
 | Human Liver |
|
| Image 5 | HEK293 Whole Cell Lysate
 | NONO was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP40716_T100 with 1:200 dilution. Western blot was performed using ARP40716_T100 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: NONO IP with ARP40716_T100 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate. |
|
| Image 6 | Human Heart
 | Rabbit Anti-NONO Antibody
Catalog Number: ARP40716
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 7 | Human Heart
 | Rabbit Anti-NONO Antibody
Catalog Number: ARP40716
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
