ACTB Antibody - middle region (ARP40174_P050)

• Product Number: ARP40174_P050
• Name: ACTB Antibody - middle region (ARP40174_P050)
• Protein Size (# AA): 375 amino acids
• Molecular Weight: 42kDa
• NCBI Gene Id: 60
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Actin, beta
• Alias Symbols: BRWS1, PS1TP5BP1
• Peptide Sequence: Synthetic peptide located within the following region: TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
• Protein Interactions: FUS; DSTN; CAP2; CFL2; CFL1; ACTG1; ACTB; PA2G4; EHHADH; WDYHV1; HDAC6; ISG15; TP53; CEP250; TUBGCP2; TUBGCP3; VCP; UBC; TUBG1; SUMO2; LGALS3BP; AMOT; NEDD1; HAUS1; IVNS1ABP; STAU1; MDM2; CORO7; LGR4; ASB12; ASB9; ZBTB1; ERG; RPA3; RPA2; RPA1; CDKN2A; PAR
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml.
• Blocking Peptide: For anti-ACTB (ARP40174_P050) antibody is Catalog # AAP40174
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human ACTB
• Uniprot ID: P60709
• Protein Name: Actin, cytoplasmic 1
• Publications:

  Regular exercise modulates cardiac mast cell activation in ovariectomized rats. J Physiol Sci. 66, 165-73 (2016). 26467449

• Sample Type Confirmation:

  ACTB is strongly supported by BioGPS gene expression data to be expressed in HeLa, HepG2, Jurkat, MCF7

• Protein Accession #: NP_001092
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001101
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: ACTB
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Pig, Sheep, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Goat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Image 1: Human HepG2
  
  Host: Rabbit
  Target Name: ACTB
  Sample Tissue: Human HepG2
  Antibody Dilution: 1.0ug/ml
• Image 2: Mouse Heart
  
  Host: Mouse
  Target Name: ACTB
  Sample Tissue: Mouse Heart
  Antibody Dilution: 1ug/ml
• Image 3: Mouse Testis
  
  Host: Mouse
  Target Name: ACTG1
  Sample Tissue: Mouse Testis
  Antibody Dilution: 1ug/ml
• Image 4: Mouse Heart
  
  Host: Rabbit
  Target Name: ACTB
  Sample Tissue: Mouse Heart
  Antibody Dilution: 1ug/ml
• Image 5: Human Appendix (Colon)
  
  Rabbit Anti-ACTB Antibody
  Catalog Number: ARP40174_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue
  Observed Staining: Cytoplasm
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 6: Human
  
  1. The sample 1 - 8 is used N2 wild type C. elegans.
  2. Each lane contain 60 ug of sample and the loading volume is 36 ul.
  3. The number 1 sample is a control without any treatment.
  4. As I get 50 ug of primary Ab, I diluted with 50 ul of water. I use 5 ul each time in 10 ml of 5% milk in PBST buffer.
  5. The secondary antibody for chemiluminescence is Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) (111-035-003) from Jacksonimmunoresearch; for the chromogenic detection, I used goat anti-rabbit IgG-Ap (sc-2007) from santa cruz.
• Image 7: Human
  
  Sample type: 1-8.N2 WT C elegans (60ug)Primary Dilution: 1:2000Secondary Antibody: goat anti-Rabbit HRPSecondary Dilution: 1:2000
• Image 8: Human Breast
  
  IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml.
• Image 9: Human brain
  
  Sample Type:
  Human brain stem cells
  Primary Antibody Dilution:
  1:500
  Secondary Antibody:
  Goat anti-rabbit Alexa-Fluor 594
  Secondary Antibody Dilution:
  1:1000
  Color/Signal Descriptions:
  ACTB: Red DAPI:Blue
  Gene Name:
  ACTB
  Submitted by:
  Dr. Yuzhi Chen, University of Arkansas for Medical Science