TRPV4 Antibody - middle region (ARP35330_P050)

• Product Number: ARP35330_P050
• Name: TRPV4 Antibody - middle region (ARP35330_P050)
• Protein Size (# AA): 811 amino acids
• Molecular Weight: 91kDa
• NCBI Gene Id: 59341
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Transient receptor potential cation channel, subfamily V, member 4
• Alias Symbols: SMAL, VRL2, BCYM3, CMT2C, SPSMA, TRP12, VROAC, HMSN2C, OTRPC4, SSQTL1
• Peptide Sequence: Synthetic peptide located within the following region: RVDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Corey,D.P. (er) Pflugers Arch. (2008) In press
• Description of Target: TRPV4 is a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation of TRPV4 by exposure to hypotonicity within the physiological range exhibits an outward rectification. TRPV4 also activated by low pH, citrate and phorbol esters and increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3) This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene.
• Protein Interactions: UBC; ARRB2; KRIT1; ITCH; PACSIN3; PACSIN1; TRPV4; PACSIN2; MAP7; SRC; LYN; YES1; LCK; HCK; FYN; CALM1; OS9; PACS1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TRPV4 (ARP35330_P050) antibody is Catalog # AAP35330 (Previous Catalog # AAPP06566)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRPV4
• Uniprot ID: Q9HBA0-2
• Protein Name: Transient receptor potential cation channel subfamily V member 4
• Protein Accession #: NP_671737
• Purification: Affinity Purified
• Nucleotide Accession #: NM_147204
• Tested Species Reactivity: Human, Rat
• Gene Symbol: TRPV4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 86%; Guinea Pig: 86%; Human: 100%; Mouse: 86%; Pig: 93%; Rabbit: 100%; Rat: 93%
• Image 1: Human ACHN
  
  WB Suggested Anti-TRPV4 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:12500
  Positive Control: ACHN cell lysate
• Image 2: Rat Hippocamus
  
  TRPV4 Rat Hippocampus We tested the antibodies in Immunohistochemistry studies. The dilutions for all the above primary antibodies were 1:500 and the same for the secondary antibody, Alexa Fluor 568-conjugated goat anti-rabbit antibodies (Invitrogen).