KEAP1 Antibody - C-terminal region (ARP34728_T100)

• Product Number: ARP34728_T100
• Name: KEAP1 Antibody - C-terminal region (ARP34728_T100)
• Protein Size (# AA): 624 amino acids
• Molecular Weight: 70kDa
• NCBI Gene Id: 9817
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Kelch-like ECH-associated protein 1
• Alias Symbols: INrf2, KLHL19
• Peptide Sequence: Synthetic peptide located within the following region: TWTFVAPMKHRRSALGITVHQGRIYVLGGYDGHTFLDSVECYDPDTDTWS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Padmanabhan,B., (2006) Mol. Cell 21 (5), 689-700
• Description of Target: KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
• Protein Interactions: OXR1; ZSCAN32; LSM3; KLHL3; FBXW11; MAPKBP1; NUDT4; DPP3; RBX1; ATG5; SQSTM1; PTMA; PRKAG1; NFE2L2; MAD2L1; GYPA; ETF1; BCL2; CUL3; RECK; UBC; PGAM5; FAM117B; WDR83; CCNB1IP1; HSP90AA1; XPO1; MCMBP; KEAP1; SLK; MCM3; IKBKB; AMER1; GPRASP2; PRKCE; NFE2L1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-KEAP1 (ARP34728_T100) antibody is Catalog # AAP34728 (Previous Catalog # AAPP23660)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KEAP1
• Uniprot ID: Q14145
• Protein Name: Kelch-like ECH-associated protein 1
• Publications:

  Protein levels in Keap1-Nrf2 system in human failing heart. Int. J. Cardiol. 225, 62-64 (2016). 27710805

• Sample Type Confirmation:

  KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_987096
• Purification: Protein A purified
• Nucleotide Accession #: NM_203500
• Tested Species Reactivity: Human
• Gene Symbol: KEAP1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Image 1: Human kidney
  
  Human kidney
• Image 2: HepG2
  
  Host: Rabbit
  Target Name: KEAP1
  Sample Type: HepG2
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 1.25ug/mL
  Peptide Concentration: 1.0ug/mL
  Lysate Quantity: 25ug/lane
  Gel Concentration: 12%KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2
• Image 3: Human HepG2
  
  WB Suggested Anti-KEAP1 Antibody Titration: 1.0ug/ml
  Positive Control: HepG2 cell lysateKEAP1 is supported by BioGPS gene expression data to be expressed in HepG2
• Image 4: Human HCT116 Whole Cell
  
  Host: Rabbit
  Target Name: KEAP1
  Sample Tissue: Human HCT116 Whole Cell
  Antibody Dilution: 1ug/ml