MBD2 Antibody - N-terminal region (ARP32485_P050)

• Product Number: ARP32485_P050
• Name: MBD2 Antibody - N-terminal region (ARP32485_P050)
• Protein Size (# AA): 302 amino acids
• Molecular Weight: 32kDa
• NCBI Gene Id: 8932
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Methyl-CpG binding domain protein 2
• Alias Symbols: DMTase, NY-CO-41
• Peptide Sequence: Synthetic peptide located within the following region: RAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Xing,J., (2008) Br. J. Cancer 98 (10), 1716-1722
• Description of Target: MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation signal.It is also reported that the MBD2 functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
• Protein Interactions: UBC; SOX2; HDAC2; HDAC1; MTA3; LAMTOR3; TFAP4; KDM5B; CDK2AP1; ESR1; KDM1A; MTA2; MBD2; RBBP7; RBBP4; CHD3; tat; MBD3L2; MBD3; csul; GATAD2A; TACC3; PRMT5; WDR77; PRMT1; PTK2B; TRIM27; GATAD2B; MBD3L1; GPN1; SIN3A; HINFP; DHX9; CREBBP; DNMT1; tax; NEWENTR
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-MBD2 (ARP32485_P050) antibody is Catalog # AAP32485 (Previous Catalog # AAPP03484)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MBD2
• Uniprot ID: Q9UBB5
• Protein Name: Methyl-CpG-binding domain protein 2
• Publications:

  Jungwirth, M., Dear, M. L., Brown, P., Holbrook, K. & Goodchild, R. Relative tissue expression of homologous torsinB correlates with the neuronal specific importance of DYT1 dystonia-associated torsinA. Hum. Mol. Genet. 19, 888-900 (2010). 20034025

• Sample Type Confirmation:

  MBD2 is strongly supported by BioGPS gene expression data to be expressed in 721_B

• Protein Accession #: NP_056647
• Purification: Affinity Purified
• Nucleotide Accession #: NM_015832
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: MBD2
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Mouse: 100%; Rat: 100%
• Image 1: Human 721_B
  
  WB Suggested Anti-MBD2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:12500
  Positive Control: 721_B cell lysateMBD2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
• Image 2: liver
  
  Rabbit Anti-MBD2 Antibody
  Catalog Number: ARP32485_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Adult liver
  Observed Staining: Nuclear
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy2/3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  Protocol located in Reviews and Data.
• Image 3: Mouse ES
  
  Lanes:
  Lane 1: 15ug WT mouse ES lysate
  Lane 2: 15ug MBD2 KO mouse ES lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Goat anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:2500
  Gene Name:
  MBD2 a
  Submitted by:
  Austin J. Cooney, Baylor College of Medicine