TIAL1 Antibody - C-terminal region (AVARP02048_T100)

• Product Number: AVARP02048_T100
• Name: TIAL1 Antibody - C-terminal region (AVARP02048_T100)
• Protein Size (# AA): 375 amino acids
• Molecular Weight: 42 kDa
• NCBI Gene Id: 7073
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: TIA1 cytotoxic granule-associated RNA binding protein-like 1
• Alias Symbols: TCBP, TIAR
• Peptide Sequence: Synthetic peptide located within the following region: WNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIPPPNQAGYGMASYQTQ
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Esclatine,A., et al., (2004) J. Virol. 78 (16), 8582-8592
• Description of Target: The protein encoded by TIAL1 is a member of a family of RNA-binding proteins and possesses nucleolytic activity against cytotoxic lymphocyte target cells. The gene product is a cytotoxic granule-associated protein and has been shown to bind specifically to poly(A) homopolymers and to fragment DNA in permeabilized target cells. It has been suggested that members of this protein family may be involved in the induction of apoptosis. One isoform contains a lysosome-targeting motif in its C-terminal auxiliary domain; however, alternative splicing results in a T-cluster DNA-binding isoform, differing at the C-terminus where a hydrophobic sequence replaces the lysosome-targeting motif.
• Protein Interactions: SUMO2; UBC; RPA3; RPA2; RPA1; BMI1; SOX2; MAPK1; GADD45A; VCAM1; ITGA4; FN1; MRPL53; SARNP; UFM1; SBDS; MRPS28; TOMM40; ACAA2; GNB2L1; SF3B4; SCAMP3; SCO2; ALDH5A1; VDAC1; UBE2D2; THY1; SOD1; MBNL1; CAND1; DCUN1D1; COPS5; COPS6; CUL1; CUL2; CUL3; CUL4A; C
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-TIAL1 (AVARP02048_T100) antibody is Catalog # AAP30620 (Previous Catalog # AAPP01273)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TIAL1
• Uniprot ID: Q01085
• Protein Name: Nucleolysin TIAR
• Sample Type Confirmation:

  TIAL1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_003243
• Purification: Protein A purified
• Nucleotide Accession #: NM_003252
• Tested Species Reactivity: Human
• Gene Symbol: TIAL1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 85%; Rabbit: 100%; Rat: 85%; Zebrafish: 76%
• Image 1:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Protein may be acetylated or phosphorylated.