KRT2A Antibody - middle region (ARP41735_P050)

• Product Number: ARP41735_P050
• Name: KRT2A Antibody - middle region (ARP41735_P050)
• Protein Size (# AA): 639 amino acids
• Molecular Weight: 65 kDa
• NCBI Gene Id: 3849
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Keratin 2
• Alias Symbols: K2e, KRTE, CK-2e, KRT2A, KRT2E
• Peptide Sequence: Synthetic peptide located within the following region: EVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELN
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Bloor,B.K., (2003) Am. J. Pathol. 162 (3), 963-975
• Description of Target: KRT2A is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma.The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
• Protein Interactions: KRT40; KRT38; KRT31; KRT19; KRT13; Fbxl16; LGR4; EED; ADRB2; PAN2; Nphp4; IQCB1; VCAM1; UBC; FN1; EIF4A3; MAGOH; KRT10; KRT1; UBASH3B; SHC1; PIK3R2; INPPL1; GRB2; EPS15; CRK; AP2M1; CBL; CAND1; DCUN1D1; COPS5; COPS6; CUL1; CUL2; CUL3; CUL4A; CUL4B; CUL5;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-KRT2 (ARP41735_P050) antibody is Catalog # AAP41735 (Previous Catalog # AAPP24378)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KRT2A
• Uniprot ID: P35908
• Protein Name: Keratin, type II cytoskeletal 2 epidermal
• Protein Accession #: NP_000414
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000423
• Tested Species Reactivity: Human
• Gene Symbol: KRT2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 79%; Dog: 100%; Guinea Pig: 79%; Horse: 88%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 79%; Rat: 81%
• Image 1: Human Intestine
  
  Rabbit Anti-KRT2AAntibody
  Catalog Number: ARP41735
  Paraffin Embedded Tissue: Human Intestine
  Cellular Data: Epithelial cells of intestinal villas
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Human Skin
  
  Rabbit Anti-KRT2 antibody
  Catalog Number: ARP41735
  Formalin Fixed Paraffin Embedded Tissue: Human Skin
  Primary antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20x
  Exposure Time: 0.5-2.0sec
• Image 3: Human Lung
  
  Rabbit Anti-KRT2 Antibody
  Catalog Number: ARP41735
  Paraffin Embedded Tissue: Human alveolar cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 4:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The protein may be modified by phosphorylation.