| Product Number |
ARP40691_P050 |
| Product Page |
www.avivasysbio.com/sfrs1-antibody-c-terminal-region-arp40691-p050.html |
| Name |
SFRS1 Antibody - C-terminal region (ARP40691_P050) |
| Protein Size (# AA) |
248 amino acids |
| Molecular Weight |
27kDa |
| NCBI Gene Id |
6426 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Serine/arginine-rich splicing factor 1 |
| Alias Symbols |
ASF, SF2, SFRS1, SF2p33, SRp30a |
| Peptide Sequence |
Synthetic peptide located within the following region: EFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSR |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Takagaki,Y., (2005) J. Biol. Chem. 280 (50), 41761-41768 |
| Description of Target |
SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene.Alternative mRNA splicing plays an important role in development and differentiation; many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2; MIM 600813), have been extensively characterized.Alternative mRNA splicing plays an important role in development and differentiation; many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2; MIM 600813), have been extensively characterized (Bermingham et al., 1995).[supplied by OMIM]. |
| Protein Interactions |
TRAF3IP2; HUWE1; SPRTN; STAU1; UBC; MDM2; ZBTB1; RNF2; EZH2; EED; SUZ12; PARK2; SRPK2; SRPK1; SRPK3; FBXO6; YWHAE; U2AF2; PAN2; MPG; CIR1; FN1; CDK6; HNRNPA0; RNPS1; SRSF10; NXF1; PRPF4; BCL10; U2AF1; SRSF4; MAGOH; SMURF1; EIF4A3; ESR1; ECT2; BRCA1; BARD1 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SRSF1 (ARP40691_P050) antibody |
| Blocking Peptide |
For anti-SRSF1 (ARP40691_P050) antibody is Catalog # AAP40691 (Previous Catalog # AAPP10434) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human SFRS1 |
| Uniprot ID |
Q07955 |
| Protein Name |
Serine/arginine-rich splicing factor 1 |
| Publications |
Li, J. et al. A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells. FEBS Lett. 584, 3198-202 (2010). 20547158 |
| Sample Type Confirmation |
There is BioGPS gene expression data showing that SRSF1 is expressed in HepG2 |
| Protein Accession # |
NP_008855 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_006924 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
SRSF1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human HepG2
 | WB Suggested Anti-SFRS1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that SRSF1 is expressed in HepG2 |
|
| Image 2 | Human heart
 | Rabbit Anti-SRSF1 Antibody
Catalog Number: ARP40691_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
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