| Product Number |
ARP40568_T100 |
| Product Page |
www.avivasysbio.com/pcbp2-antibody-middle-region-arp40568-t100.html |
| Name |
PCBP2 Antibody - middle region (ARP40568_T100) |
| Protein Size (# AA) |
366 amino acids |
| Molecular Weight |
40kDa |
| NCBI Gene Id |
5094 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Poly(rC) binding protein 2 |
| Alias Symbols |
HNRPE2, HNRNPE2, hnRNP-E2 |
| Peptide Sequence |
Synthetic peptide located within the following region: VIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQ |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Oh,J.H., (2005) Mamm. Genome 16 (12), 942-954 |
| Description of Target |
PCBP2 appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. This protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes.The protein encoded by this gene appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1 intronless gene which has similar functions. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. It also has two processed pseudogenes PCBP2P1 and PCBP2P2. There are presently two alternatively spliced transcript variants described for this gene. |
| Protein Interactions |
FUS; HUWE1; UBC; SNRPA; SUMO2; SUMO3; CEP250; MDM2; EED; TARDBP; ADRB2; IGSF8; HMGA1; CD81; HMGA2; WDR83; SLU7; CASK; UBL4A; VCAM1; PCBP2; NOS2; ITGA4; FN1; BRCA1; PRPF40A; SF3B2; NKAP; WDR77; PAXIP1; BARD1; PPIB; EEF1A1; FBXO6; UBD; PCBP1; QKI; CDK2; COP |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PCBP2 (ARP40568_T100) antibody |
| Blocking Peptide |
For anti-PCBP2 (ARP40568_T100) antibody is Catalog # AAP40568 (Previous Catalog # AAPP22746) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human PCBP2 |
| Uniprot ID |
Q6IPF4 |
| Protein Name |
Poly(rC)-binding protein 2 |
| Publications |
Han, W. et al. RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3. J. Clin. Invest. 123, 2103-18 (2013). 23585479
Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion. Oncotarget. 7, 19483-98 (2016). 26761212 |
| Sample Type Confirmation |
There is BioGPS gene expression data showing that PCBP2 is expressed in Jurkat |
| Protein Accession # |
NP_005007 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_005016 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
PCBP2 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Guinea Pig, Horse |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100% |
| Image 1 | Human Lung
 | Rabbit Anti-PCBP2 Antibody
Catalog Number: ARP40568
Paraffin Embedded Tissue: Human Lung
Cellular Data: Alveolar cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 2 | Human Jurkat
 | Host: Rabbit
Target Name: PCBP2
Sample Tissue: Human Jurkat
Antibody Dilution: 1.0ug/ml |
|
| Image 3 | Mouse Pancreas
 | Host: Mouse
Target Name: PCBP2
Sample Tissue: Mouse Pancreas
Antibody Dilution: 1ug/ml |
|
