| Product Number |
ARP40185_T100 |
| Product Page |
www.avivasysbio.com/prmt5-antibody-n-terminal-region-arp40185-t100.html |
| Name |
PRMT5 Antibody - N-terminal region (ARP40185_T100) |
| Protein Size (# AA) |
620 amino acids |
| Molecular Weight |
68kDa |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
10419 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Protein arginine methyltransferase 5 |
| Alias Symbols |
HSL7, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5 |
| Peptide Sequence |
Synthetic peptide located within the following region: FDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Azzouz,T.N., (2005) J. Biol. Chem. 280 (41), 34435-34440 |
| Description of Target |
PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription. |
| Protein Interactions |
UBC; TP53; ING2; PRDM1; NFE4; WDR5; SUMO2; WWOX; CDYL2; LENG8; LDHAL6B; PRPF38A; ING5; ZNF436; WDR77; GRHL3; TRIB3; RSRP1; LUC7L; RBM23; GTPBP2; H3F3C; FOXK1; ZUFSP; FAM76B; NUDCD2; MAGEB2; NELFCD; COPS4; COPS7A; DCAF8; SPAG8; SLU7; MCRS1; CUL4A; ZNF2; YW |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PRMT5 (ARP40185_T100) antibody |
| Blocking Peptide |
For anti-PRMT5 (ARP40185_T100) antibody is Catalog # AAP40185 (Previous Catalog # AAPS01710) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT5 |
| Uniprot ID |
O14744 |
| Protein Name |
Protein arginine N-methyltransferase 5 |
| Sample Type Confirmation |
PRMT5 is supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_001034708 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_001039619 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
PRMT5 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-PRMT5 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysatePRMT5 is supported by BioGPS gene expression data to be expressed in Jurkat |
|
| Image 2 | Human Kidney
 | Rabbit Anti-PRMT5 Antibody
Catalog Number: ARP40185
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human Lung
 | Rabbit Anti-PRMT5 Antibody
Catalog Number: ARP40185
Paraffin Embedded Tissue: Human Lung
Cellular Data: Alveolar cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
