TRIM31 Antibody - N-terminal region (ARP34717_P050)

• Product Number: ARP34717_P050
• Name: TRIM31 Antibody - N-terminal region (ARP34717_P050)
• Protein Size (# AA): 425 amino acids
• Molecular Weight: 48 kDa
• NCBI Gene Id: 11074
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Tripartite motif containing 31
• Alias Symbols: RNF, HCG1, HCGI, C6orf13
• Peptide Sequence: Synthetic peptide located within the following region: CRESKDHKSHNVSLIEEAAQNYQGQIQEQIQVLQQKEKETVQVKAQGVHR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Reymond,A., et al., (2001) EMBO J. 20(9), 2140-51
• Description of Target: TRIM31 encodes for a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to both the cytoplasm and the nucleus.
• Protein Interactions: MAGEA2B; MAGEA1; USP7; MKRN3; TRIM27; MNAT1; UBE2E2; UBE2D3; UBE2D2; UBE2D1; ZSCAN1; IKBKG; UBE2W; UBE2D4; UBE2K; TRIM31; TRAF2; TRIM23;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: SPR Affinity Characterization
  
• Blocking Peptide: For anti-TRIM31 (ARP34717_P050) antibody is Catalog # AAP34717 (Previous Catalog # AAPP05911)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM31
• Uniprot ID: Q9BZY9
• Protein Name: E3 ubiquitin-protein ligase TRIM31
• Protein Accession #: NP_008959
• Purification: Affinity Purified
• Nucleotide Accession #: NM_007028
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: TRIM31
• Predicted Species Reactivity: Human, Mouse, Cow, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 79%; Human: 100%; Mouse: 86%; Rabbit: 93%
• Image 1: Mouse Heart
  
  Host: Mouse
  Target Name: TRIM31
  Sample Tissue: Mouse Heart
  Antibody Dilution: 1ug/ml
• Image 2: Human kidney
  
  Human kidney
• Image 3: Human Jurkat
  
  WB Suggested Anti-TRIM31 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:12500
  Positive Control: Jurkat cell lysate
• Image 4:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.