| Product Number |
P100861_P050 |
| Product Page |
www.avivasysbio.com/rara-antibody-middle-region-p100861-p050.html |
| Name |
RARA Antibody - middle region (P100861_P050) |
| Protein Size (# AA) |
462 amino acids |
| Molecular Weight |
51kDa |
| NCBI Gene Id |
5914 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Retinoic acid receptor, alpha |
| Description |
|
| Alias Symbols |
RAR, NR1B1 |
| Peptide Sequence |
Synthetic peptide located within the following region: EMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSSPATHS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Garapaty,S., (2008) J. Biol. Chem. 283 (11), 6806-6816 |
| Description of Target |
Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor, which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1, SMRT, and histone deacetylase. When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases, and the basic transcription machinery. |
| Protein Interactions |
NCOR2; NCOR1; RXRG; MED1; HDAC1; NRIP1; TEKT4; NSD1; NCOA1; CEBPA; ITGB1BP2; SUMO1; UBC; MBD3; CBX5; MTA2; E2F1; WWOX; Sumo2; RXRB; RXRA; AKT1; MMS19; Dlg4; TMEM54; FN1; USP7; NCOA2; SUZ12; EZH2; PML; PSMC5; FAS; PRAM1; TBL1XR1; PRAME; ARID5A; UBE3A; UBQL |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-RARA (P100861_P050) antibody |
| Blocking Peptide |
For anti-RARA (P100861_P050) antibody is Catalog # AAP31224 (Previous Catalog # AAPP01969) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human RARA |
| Uniprot ID |
P10276 |
| Protein Name |
Retinoic acid receptor alpha |
| Sample Type Confirmation |
RARA is supported by BioGPS gene expression data to be expressed in OVCAR3 |
| Protein Accession # |
NP_000955 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_000964 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
RARA |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Rabbit |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 85%; Rabbit: 100%; Rat: 85% |
| Image 1 | Human OVCAR-3
 | WB Suggested Anti-RARA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: OVCAR-3 cell lysateRARA is supported by BioGPS gene expression data to be expressed in OVCAR3 |
|
| Image 2 | Human liver
 | WB Suggested Anti-RARA antibody Titration: 1 ug/mL
Sample Type: Human liver |
|
| Image 3 | liver
 | Rabbit Anti-RARA Antibody
Catalog Number: P100861_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult liver
Observed Staining: Cytoplasmic (abundant), Nuclear (very rare)
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 2.0 sec
Protocol located in Reviews and Data. |
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