| Product Number |
OORA00134 |
| Product Page |
www.avivasysbio.com/avidin-fluorescein-conjugated-oora00134.html |
| Name |
AVIDIN Fluorescein Conjugated (OORA00134) |
| Molecular Weight |
390 |
| Conjugation |
FITC |
| Concentration |
1.1 mg/ml |
| Alias Symbols |
FITC |
| Product Format |
Lyophilized |
| Description of Target |
Avidin |
| Reconstitution and Storage |
2°C to 8°C, -20°C or -80°C |
| Datasheets/Manuals |
Printable datasheet for OORA00134 |
| Specificity |
Avidin Fluorescein Conjugated was prepared from chromatographical pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-Avidin. No reaction was observed against anti-Streptavidin. |
| Additional Information |
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Color: Green
Conjugation Reference: Modified from The and Feltkamp, Immunology 18; 865, 1970.
Emission Wavelength: 528
Excitation Wavelength: 495
Fluorochrome/Protein Ratio: 2.0 moles FITC per mole of Avidin |
| Additional Information |
Label: Fluorescein (FITC)
Preservative: 0.01% (w/v) Sodium Azide
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Application Info |
Avidin Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.
All assays should be optimized by the user. Recommneded dilutions may be listed below:
FLISA: 1:10,000 - 1:50,000 IF: 1:1,000 - 1:5,000 |
| Additional Information |
Description: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Uniprot ID |
https://www.uniprot.org/uniprot/P02701 |
| Protein Name |
P02701 |
| Predicted Species Reactivity |
Avidin, Gallus gallus |
| Application |
Biological activity assay, Dot blot, Flow cytometry, Fluorescence-linked immunosorbent assay, Immunofluorescence, Immunoprecipitation, Western blot |
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