| Product Number |
OOPA00156 |
| Product Page |
www.avivasysbio.com/ada-protein-oopa00156.html |
| Name |
ADA Protein (OOPA00156) |
| Protein Size (# AA) |
Recombinant amino acids |
| Host |
Escherichia Coli. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Alias Symbols |
Adenosine deaminase, Adenosine aminohydrolase, ADA1, EC 3.5.4.4. |
| Peptide Sequence |
MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL |
| Product Format |
ADA is supplied as a sterile filtered clear solution. |
| Reconstitution and Storage |
Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OOPA00156 |
| Formulation |
ADA protein 0.5mg/ml is supplied in 20mM Tris-HCL, pH-8, 1mM DTT and 20% Glycerol. |
| Biological Activity |
Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C. |
| Additional Information |
Product Introduction: ADA catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. ADA has a vital part in purine metabolism and in adenosine homeostasis. ADA performs as a positive regulator of T-cell coactivation, by binding DPP4 which regulates lymphocyte-epithelial cell adhesion.
Product Description: ADA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 383 amino acids (1-363) and having a molecular mass of 42.9 kDa. The ADA is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
| Purification |
Greater than 85.0% as determined by SDS-PAGE. |
| Gene Symbol |
ADA |
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