Product Number |
OKCD00643 |
Product Page |
www.avivasysbio.com/mpc1-elisa-kit-human-okcd00643.html |
Name |
MPC1 ELISA Kit (Human) (OKCD00643) |
Protein Size (# AA) |
109 amino acids |
NCBI Gene Id |
51660 |
Gene Full Name |
mitochondrial pyruvate carrier 1 |
Alias Symbols |
brain protein 44-like protein;BRP44L;CGI-129;HSPC040 protein;mitochondrial pyruvate carrier 1;MPYCD;SLC54A1. |
Description of Target |
Mediates the uptake of pyruvate into mitochondria. |
Reconstitution and Storage |
2°C to 8°C|-20°C |
Datasheets/Manuals |
Printable datasheet for MPC1 ELISA Kit (Human) (OKCD00643) |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Mitochondrial Pyruvate Carrier Protein 1 (MPC1).
No significant cross-reactivity or interference between Mitochondrial Pyruvate Carrier Protein 1 (MPC1) and analogues was observed. |
Assay Info |
Assay Methodology: Quantitative Sandwich Immunoassay |
Sensitivity |
< 0.073 ng/mL |
Uniprot ID |
Q9Y5U8 |
Protein Name |
Mitochondrial pyruvate carrier 1 |
Protein Accession # |
NP_057182 |
Sample Type |
Tissue homogenates and other biological fluids. |
Nucleotide Accession # |
NM_016098.3 |
ELISA Kit Component |
Component | Amount |
Anti-MPC1 Microplate | 96 Wells (12 x 8 Well Strips) |
MPC1 Lyophilized Standard | 2 x 10 ng |
100X Biotinylated MPC1 Detector Antibody | 120 uL |
Avidin/HRP Conjugate | 120 uL |
Standard Diluent | 1 x 20 mL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
30X Wash Buffer | 1 x 20 mL |
TMB Substrate | 1 x 9 mL |
Stop Solution | 1 x 6 mL |
|
ELISA Kit Reproducibility |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitochondrial Pyruvate Carrier Protein 1 (MPC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitochondrial Pyruvate Carrier Protein 1 (MPC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
ELISA Kit Range |
0.312-20ng/mL |
ELISA Kit Duration |
3h |
ELISA Kit Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Pyruvate Carrier Protein 1 (MPC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Pyruvate Carrier Protein 1 (MPC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Pyruvate Carrier Protein 1 (MPC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mitochondrial Pyruvate Carrier Protein 1 (MPC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
ELISA Kit Detection Method |
Colorimetric |
Gene Symbol |
MPC1 |
Bulk Note |
Please contact customer service when ordering 10+ kits. |
Predicted Species Reactivity |
Homo sapiens|Human |
Application |
Enzyme-linked Immunosorbent assay-Sandwich |
Image 1 | MPC1 ELISA
| Human MPC1 ELISA Kit standard curve |
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