Goat Anti-Rabbit IgM Antibody - PE Conjugated (OASB01958)

Data Sheet
 
Product Number OASB01958
Product Page www.avivasysbio.com/goat-anti-rabbit-igm-antibody-pe-oasb01958.html
Name Goat Anti-Rabbit IgM Antibody - PE Conjugated (OASB01958)
Isotype IgG
Conjugation R-PE
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/mL
Gene Full Name Ig mu chain C region
Reconstitution and Storage Store at 2-8C
Storage - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) and rhodamine (TRITC) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3
Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Other Applications Data Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Datasheets/Manuals Printable datasheet for Goat Anti-Rabbit IgM Antibody - PE Conjugated (OASB01958)
Specificity IgM
Additional Information Description: Pooled antisera from goats hyperimmunized with rabbit IgM; purified by affinity chromatography on pooled rabbit IgM covalently linked to agarose; reacts with the heavy chain of rabbit IgM; cross-adsorbed against rabbit IgG for minimal reactivity
Application Info Direct and indirect immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunoblotting, Immunohistochemistry
Immunogen Rabbit IgM
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution
FLISA:

TRITC conjugate 1:100-1:400
Cross Absorption Rabbit IgG
Characterization To ensure lot- to- lot consistency, each batch of product is tested by ELISA or Fluorescent ELISA forconformance to characteristics of a standard reference reagent.
Uniprot ID G1T5L0
Purification Affinity chromatography on rabbit IgM covalently linked to agarose
Tested Species Reactivity Rabbit
Predicted Species Reactivity Rabbit
Application FLISA, FC
Image 1
Purified rabbit IgM and IgG
FLISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-PE.
 

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