Product Number |
OASB01604 |
Product Page |
www.avivasysbio.com/goat-anti-mouse-igg2b-human-ads-antibody-oasb01604.html |
Name |
Goat Anti-Mouse IgG2b Antibody (OASB01604) |
Isotype |
IgG |
NCBI Gene Id |
16016 |
Host |
Goat |
Clonality |
Polyclonal |
Concentration |
1.0 mg/mL |
Gene Full Name |
Ig gamma-2B chain C region |
Alias Symbols |
IgG2, IgG2b, Igh-3, gamma2b |
Reconstitution and Storage |
Store at 2-8C |
Storage |
- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates aresupplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), and Allophycocyanin- Cyanine 7(APC- CY 7) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
Other Applications Data |
Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
Datasheets/Manuals |
Printable datasheet for anti-Igh-3 (OASB01604) antibody |
Specificity |
IgG2b |
Additional Information |
Description: Pooled antisera from goats hyperimmunized with mouse IgG2b; purified by affinity chromatography on mouse IgG2b covalently linked to agarose; reacts with the heavy chain of mouse IgG2b; cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human, bovine, and horse serum proteins (SP) for minimal reactivity |
Application Info |
Indirect immunostaining in conjunction with mouse IgG2b monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting |
Other Applications Image 1 Data |
Amount Used: ?1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After |
Immunogen |
Mouse IgG2b |
Warning |
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Dilution |
Immunofluorescence: FLISA: ELISA: FITC and BIOT conjugates R-PE, APC, R-PE-CY7, APC-CY7, CY5 and SPRD conjugates TRITC and TXRD conjugates AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 0.1 ug/106 cells 1:100-1:400 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
Cross Absorption |
Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified humanparaproteins. |
Characterization |
To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent. |
Uniprot ID |
P01867 |
Purification |
Affinity chromatography on mouse IgG2b covalently linked to agarose |
Tested Species Reactivity |
Mouse |
Gene Symbol |
IGHG2B |
Application |
FC, IHC-F, IHC-P, ICC, WB, ELISPOT |
Image 1 | Human peripheral blood mononuclear
| Amount Used: ?1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After washing the cells were then stained with goat anti-mouse IgG2b-FITC, following which lymphocytes were gated and analyzed by flow cytometry. |
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