Mouse CD117/c-kit Antibody : APC (OASB00656)

• Product Number: OASB00656
• Name: Mouse CD117/c-kit Antibody : APC (OASB00656)
• Clone: 2B8
• Isotype: IgG2b
• Conjugation: APC
• NCBI Gene Id: 16590
• Host: Rat
• Clonality: Monoclonal
• Concentration: 0.1 mg/mL
• Gene Full Name: Mast/stem cell growth factor receptor Kit
• Alias Symbols: W, Bs, SC, SO, Fdc, Ssm, Gsfs, SCO1, SCO5, SOW3, Tr-k, c-KI, CD117, belly, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3
• Reference: 1. Broudy, V.C., et al, 1996. Blood 88:75
  2. Veiby, O.P., S. D. Lyman and S. E. Jacobsen. 1996. Blood 88:1256
  3. Sudo, T., et al. 1995. Oncogene 11:2469
  4. Yoshida, H., et al. 1996. Development 122: 1207
  5. Filip, S., J. Mokry, J. Vavrova, D. Cizkova, Z. Sinkorova, V. Tosnerova, and M. Blaha. 2009. Transfus. Apher. Sci 41:183
  6. Min, H., E. Montecino- Rodriguez, and K. Dorshkind. 2004. J. Immunol 173:245.
• Description of Target: The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Reconstitution and Storage: Store at 2-8C
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) , Allophycocyanin- Cyanine 5.5 (APC- CY 5.5) and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C
  - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
• Other Applications Data: Since applications vary, you should determine the optimum working dilution of theproduct that is appropriate for your specific need.
• Specificity: CD117
• Additional Information: Description: The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult. Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. SCF/c-Kit also participates in erythropoiesis in both the bone marrow and spleen. Loss-of-function mutations for SCF/c-Kit lead to a variety of pleiotropic developmental defects while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer. Examples of the former are mast cell deficiency and severe macrocytic anemia while the latter mutation has been identified in mastocytomas.
• Application Info: Flow cytometry 6, Cell homing studies 5, Hematopoiesis and development studies 5
• Other Applications Image 1 Data: Amount Used: 0.03 Ug/106 cells BALB/c bone marrow cells were double-stained with rat anti-mouse CD117-R-PE and rat anti-mouse Ly-6G-FITC, following which total cells were analyzed by flow cytometry.
• Immunogen: Mouse IL-3-dependent bone marrow mast cells
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Fluorescein conjugate
  Biotin conjugate
  R-phycoerythrin conjugate
  Allophycocyanin conjugate
  Spectral Red conjugate
  Cyanine 5 conjugate
  Allophycocyanin -Cyanine 5.5 conjugate
  Allophycocyanin -Cyanine 7 conjugate
  <= 1 ug/10⁶ cells
  <= 1 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
• Characterization: To ensure lot- to- lot consistency, each batch of product is tested for conformance to characteristics of astandard reference reagent by flow cytometry.
• Uniprot ID: P05532
• Protein Name: mast/stem cell growth factor receptor Kit
• Protein Accession #: NP_001116205.1?
• Nucleotide Accession #: NM_001122733.1
• Tested Species Reactivity: Mouse
• Gene Symbol: KIT
• Predicted Species Reactivity: Mouse
• Application: FC
• Image 1: BALB/c mouse bone marrow cells
  
  BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse CD117-APC and Rat Anti-Mouse CD45R-FITC.
• Image 2: BALB/c bone marrow
  
  Amount Used: 0.03 Ug/106 cells BALB/c bone marrow cells were double-stained with rat anti-mouse CD117-R-PE and rat anti-mouse Ly-6G-FITC, following which total cells were analyzed by flow cytometry.