Mouse CD117/c-kit Antibody : APC (OASB00656)

Data Sheet
 
Product Number OASB00656
Product Page www.avivasysbio.com/rat-anti-mouse-cd117-c-kit-antibody-apc-oasb00656.html
Name Mouse CD117/c-kit Antibody : APC (OASB00656)
Gene Symbol KIT
Alias Symbols W, Bs, Fdc, Ssm, SCO1, SCO5, SOW3, CD117, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3
Clone 2B8
Isotype IgG2b
Conjugation APC: Allophycocyanin
NCBI Gene Id 16590
Host Rat
Clonality Monoclonal
Concentration 0.1 mg/mL
Official Gene Full Name Mast/stem cell growth factor receptor Kit
Target Reference 1. Broudy, V.C., et al, 1996. Blood 88:75
2. Veiby, O.P., S. D. Lyman and S. E. Jacobsen. 1996. Blood 88:1256
3. Sudo, T., et al. 1995. Oncogene 11:2469
4. Yoshida, H., et al. 1996. Development 122: 1207
5. Filip, S., J. Mokry, J. Vavrova, D. Cizkova, Z. Sinkorova, V. Tosnerova, and M. Blaha. 2009. Transfus. Apher. Sci 41:183
6. Min, H., E. Montecino- Rodriguez, and K. Dorshkind. 2004. J. Immunol 173:245.
Description of Target The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Reconstitution and Storage Store at 2-8C
Storage - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD) , Allophycocyanin- Cyanine 5.5 (APC- CY 5.5) and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C
- The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Other Applications Data Since applications vary, you should determine the optimum working dilution of theproduct that is appropriate for your specific need.
Datasheets/Manuals Printable datasheet for anti-KIT (OASB00656) antibody
Specificity CD117
Additional Information Description: The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult. Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. SCF/c-Kit also participates in erythropoiesis in both the bone marrow and spleen. Loss-of-function mutations for SCF/c-Kit lead to a variety of pleiotropic developmental defects while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer. Examples of the former are mast cell deficiency and severe macrocytic anemia while the latter mutation has been identified in mastocytomas.
Application Info Flow cytometry 6, Cell homing studies 5, Hematopoiesis and development studies 5
Other Applications Image 1 Data Amount Used: 0.03 Ug/106 cells BALB/c bone marrow cells were double-stained with rat anti-mouse CD117-R-PE and rat anti-mouse Ly-6G-FITC, following which total cells were analyzed by flow cytometry.
Immunogen Mouse IL-3-dependent bone marrow mast cells
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Flow Cytometry: Fluorescein conjugate
Biotin conjugate
R-phycoerythrin conjugate
Allophycocyanin conjugate
Spectral Red conjugate
Cyanine 5 conjugate
Allophycocyanin -Cyanine 5.5 conjugate
Allophycocyanin -Cyanine 7 conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
Characterization To ensure lot- to- lot consistency, each batch of product is tested for conformance to characteristics of astandard reference reagent by flow cytometry.
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express KIT
Swissprot Id P05532
Protein Name mast/stem cell growth factor receptor Kit
Protein Accession # NP_001116205.1?
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express KIT
Nucleotide Accession # NM_001122733.1
Replacement Item This antibody may replace item sc-13508 from Santa Cruz Biotechnology.
Tested Species Reactivity Mouse
Notice This product is Genway GWB-1FDD10
Application FC
Image 1
BALB/c mouse bone marrow cells
BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse CD117-APC and Rat Anti-Mouse CD45R-FITC.
Image 2
BALB/c bone marrow
Amount Used: 0.03 Ug/106 cells BALB/c bone marrow cells were double-stained with rat anti-mouse CD117-R-PE and rat anti-mouse Ly-6G-FITC, following which total cells were analyzed by flow cytometry.
 

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