| Product Number |
OAGA02326 |
| Product Page |
www.avivasysbio.com/fubp1-antibody-oaga02326.html |
| Name |
FUBP1 Antibody (OAGA02326) |
| Molecular Weight |
68 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
8880 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1mg/ml |
| Gene Full Name |
far upstream element (FUSE) binding protein 1 |
| Alias Symbols |
FBP, FUBP, hDH V |
| Product Format |
Liquid PBS with 20% glycerol (pH 7) and 0.01% Thimerosal |
| Description of Target |
This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-FUBP1 (OAGA02326) antibody |
| Additional Information |
Positive Controls: HeLa, HeLa nuclear extract, mouse brain, rat brain
Cellular Localization: Nucleus |
| Application Info |
ICC/IF: 1:100~1000
IHC-FFPE: 1:100~1000
IP: 1:100~500
WB: 1:1000~10000 |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary. |
| Uniprot ID |
Q96AE4 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
FUBP1 |
| Predicted Species Reactivity |
Human, Mouse, Rat |
| Application |
IF, IHC-FFPE, IP, WB, CHIP, ICC |
| Predicted Homology Based on Immunogen Sequence |
Zebrafish (83%), Xenopus laevis (92%), Chicken (94%), Bovine (99%) |
| Image 1 | Jurkat
 | FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (OAGA02326) diluted at 1:500.
Blue: Hoechst 33342 staining.
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| Image 2 | Mouse hind brain
 | FUBP1 antibody detects FUBP1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
FUBP1 antibody (OAGA02326) dilution: 1:1000.
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| Image 3 | Mouse testis
 | FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FUBP1 antibody (OAGA02326) dilution: 1:1000.
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| Image 4 | 293T
 | FUBP-1 antibody immunoprecipitates FUBP-1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A: 30 ug whole cell lysate/extract of FUBP-1 protein expressing 293T cells B: Control with 2.5 ug of pre-immune rabbit IgG C: Immunoprecipitation of FUBP-1 by 2.5 ug of FUBP-1 antibody (OAGA02326) 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent. |
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| Image 5 | Jurkat
 | Sample (30 ug of whole cell lysate)
A: Jurkat
B: K562
C: THP-1
D: NCI-H929
7.5% SDS PAGE
OAGA02326 diluted at 1:5000
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