| Product Number |
OAGA00670 |
| Product Page |
www.avivasysbio.com/phka2-antibody-oaga00670.html |
| Name |
PHKA2 Antibody (OAGA00670) |
| Molecular Weight |
138 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
5256 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Phosphorylase b kinase regulatory subunit alpha, liver isoform |
| Alias Symbols |
PHK, PYK, XLG, PYKL, XLG2, GSD9A |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PHKA2 antibody - OAGA00670 |
| Additional Information |
Background: Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin. |
| Additional Information |
Positive Controls: HepG2 |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Synthetic peptide corresponding to a region within amino acids 1172 and 1235 of PHKA2 (Uniprot ID#P46019) |
| Additional Information |
Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
P46019 |
| Protein Name |
Phosphorylase b kinase regulatory subunit alpha, liver isoform |
| Protein Accession # |
NP_000283.1 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
PHKA2 |
| Predicted Species Reactivity |
Human |
| Application |
IHC-FFPE, WB |
| Image 1 | HepG2
 | Sample (30 ug of whole cell lysate)
A: Hep G2
5% SDS PAGE
OAGA00670 diluted at 1:500
|
| | Image 2 | Colon ca
 | Immunohistochemical analysis of paraffin-embedded Colon ca, using PHKA2(OAGA00670) antibody at 1:250 dilution. |
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