| Product Number |
OAGA00175 |
| Product Page |
www.avivasysbio.com/tubgcp2-antibody-oaga00175.html |
| Name |
TUBGCP2 Antibody (OAGA00175) |
| Molecular Weight |
103 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
10844 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
Gamma-tubulin complex component 2 |
| Alias Symbols |
ALP4, GCP2, GCP-2, SPC97, h103p, hGCP2, SPBC97, Spc97p, hSpc97, Grip103, PAMDDFS |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-TUBGCP2 antibody - OAGA00175 |
| Additional Information |
Background: Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. |
| Additional Information |
Positive Controls: A431, HeLa, NIH-3T3 |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant fragment corresponding to a region within amino acids 1 and 226 of GCP2 (Uniprot ID#Q9BSJ2) |
| Additional Information |
Cellular Localization: Centrosome |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
Q9BSJ2 |
| Protein Name |
Gamma-tubulin complex component 2 |
| Protein Accession # |
NP_006650.1 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
TUBGCP2 |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
ICC, IF, IHC-FFPE, WB |
| Image 1 | A431, Hela
 | Sample (30 ug of whole cell lysate)
A: A431
B: Hela
7.5% SDS PAGE
OAGA00175 diluted at 1:1000
|
| | Image 2 | NIH-3T3
 | Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
OAGA00175 diluted at 1:2000
|
| | Image 3 | Hepatoma
 | Immunohistochemical analysis of paraffin-embedded Hepatoma, using GCP2(OAGA00175) antibody at 1:500 dilution. |
| | Image 4 | HeLa
 | GCP2 antibody [N1N2], N-term detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term (OAGA00175) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope] |
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