FYN Antibody (OAEE00393)

• Product Number: OAEE00393
• Name: FYN Antibody (OAEE00393)
• Clone: FYN-01
• Isotype: IgG2b
• NCBI Gene Id: 2534
• Host: Mouse
• Clonality: Monoclonal
• Purity: > 95% (by SDS-PAGE)
• Concentration: 1 mg/ml
• Gene Full Name: FYN proto-oncogene, Src family tyrosine kinase
• Alias Symbols: c-syn protooncogene;FYN oncogene related to SRC, FGR, YES;OKT3-induced calcium influx regulator;p59-FYN;proto-oncogene c-Fyn;proto-oncogene Syn;SLK;src/yes-related novel;src-like kinase;SYN;tyrosine kinase p59fyn(T);tyrosine-protein kinase Fyn.
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Reference: Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinaseactivation. J Immunol. 2007 Feb 15;178(4):2262-71.
  Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20.
  Makinodan E, Manabe T, Makinodan M, Yamauchi T, Matsuyoshi H, Sakumura R, Tatsumi K, Wanaka A. A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells. Neuroscience. 2007 Jun 15;147(1):1-4.
  Samayawardhena LA, Kapur R, Craig AW: Involvement of Fyn kinase in Kit and integrin-mediated Rac activation,cytoskeletal reorganization, and chemotaxis of mast cells. Blood. 2007 May 1;109(9):3679-86.
  Filby A, Seddon B, Kleczkowska J, Salmond R, Tomlinson P, Smida M, Lindquist JA, Schraven B, Zamoyska R. Fyn regulates the duration of TCR engagement needed for commitment to effector function. J Immunol. 2007 Oct 1;179(7):4635-44.
  Wu HY, Hsu FC, Gleichman AJ, Baconguis I, Coulter DA, Lynch DR: Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2Bcleavage in neurons and heterologous systems. J Biol Chem. 2007 Jul 13;282(28):20075-87.
  Hattori K, Uchino S, Isosaka T, Maekawa M, Iyo M, Sato T, Kohsaka S, Yagi T, Yuasa S: Fyn is required for haloperidol-induced catalepsy in mice. J Biol Chem. 2006 Mar 17;281(11):7129-35.
  Talmor-Cohen A, Tomashov-Matar R, Tsai WB, Kinsey WH, Shalgi R: Fyn kinase-tubulin interaction during meiosis of rat eggs. Reproduction. 2004 Oct;128(4):387-93.
  Utting O, Priatel JJ, Teh SJ, Teh HS: p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation. J Immunol. 2001 Feb 1;166(3):1540-6.
• Description of Target: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.
• Reconstitution and Storage: 2°C to 8°C
• Specificity: The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase.
• Immunogen: Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
• Formulation: Purified
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Uniprot ID: P06241
• Protein Name: Tyrosine-protein kinase Fyn
• Protein Accession #: NP_002028.1; NP_694592.1; NP_694593.1; XP_005266947.1; XP_005266949.1
• Purification: Purified
• Nucleotide Accession #: NM_002037.5; NM_153047.3; NM_153048.3; XM_005266890.2; XM_005266892.2
• Gene Symbol: FYN
• Predicted Species Reactivity: Human|Mouse
• Application: Immunocytochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Image 1: Human Hela
  
  Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). 
• Image 2: Human primary fibroblasts
  
  Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
• Image 3: Mouse fibroblasts
  
  Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue). 
• Image 4: Human T cell lysates
  
  Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01).
  Lane 1: original lysate of T cells
  Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.