Src Antibody (Phospho-Tyr418) (OAEC00090)

• Product Number: OAEC00090
• Name: Src Antibody (Phospho-Tyr418) (OAEC00090)
• Molecular Weight: 60 kDa
• NCBI Gene Id: 6714
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Proto-oncogene tyrosine-protein kinase Src
• Alias Symbols: ASV, SRC1, THC6, c-SRC, p60-Src
• Product Format: Liquid
• Reference: Dufilho M, et al. (2005) J Biol Chem. 280(43):35999-36006. Sanders MA, et al. (2005) J Biol Chem. 280(25): 23516-23522. Murugappan S, et al. (2005) Blood. 106(2):550-557. Gu JJ, et al. (2005) Blood. 105(8): 3270-3277.
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: Src (phospho-Tyr418) antibody detects endogenous levels of Src only when phosphorylated at tyrosine 418.
• Application Info: WB: 1:500-1:1000, IHC: 1:50-1:100
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 418 (N-E-Y^P-T-A).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P12931
• Protein Name: Proto-oncogene tyrosine-protein kinase Src
• Protein Accession #: NP_005408.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Gene Symbol: SRC
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC
• Image 1: COLO205
  
  Western blot analysis of extracts from COLO205 cells using Src (Ab-418) antibody (OAEC00464, Lane 1 and 2) and Src (phospho-Tyr418) antibody (OAEC00090, Lane 3 and 4).
• Image 2: Human breast carcinoma
  
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (phospho- Tyr418) antibody (OAEC00090).