Estrogen Receptor- Alpha Antibody (Phospho-Ser167) (OAEC00072)

Data Sheet
 
Product Number OAEC00072
Product Page www.avivasysbio.com/estrogen-receptor-alpha-antibody-phospho-ser167-oaec00072.html
Name Estrogen Receptor- Alpha Antibody (Phospho-Ser167) (OAEC00072)
Molecular Weight 66 kDa
NCBI Gene Id 2099
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name Estrogen receptor
Alias Symbols ER, ESR, Era, ESRA, ESTRR, NR3A1
Product Format Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reference Shah YM, et al. (2005). Mol Endocrinol.19 (3): 732-748.
Sun M, et al. (2001). Cancer Res.61 (16): 5985-5991.
Campbell RA, et al. (2001). J Biol Chem.276 (13): 817-9824.
Chen D, et al. (2000). Mol Cell.6 1): 127-137.
Reconstitution and Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for anti-ESR1 antibody OAEC00072
Specificity Estrogen Receptor-alpha (phospho-Ser167) antibody detects endogenous levels of Estrogen Receptor-alpha only when phosphorylated at serine167.
Application Info WB 1:500~1000
IHC 1:50~100
IF 1:100~200
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-alpha around the phosphorylation site of serine 167 (L-A-SP-T-N).
Uniprot ID P03372
Protein Name Estrogen receptor
Protein Accession # NP_000116.2
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Gene Symbol ESR1
Predicted Species Reactivity Human, Mouse
Application WB, IHC, IF
Image 1
Human breast carcinoma
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-? (phospho-Ser167) antibody (OAEC00072).
Image 2
MCF-7
Western blot analysis of extracts from MCF-7 cells using Estrogen Receptor-? (phospho-Ser167) antibody (OAEC00072).
Image 3
MCF-7
Immunofluroescence staining of methanol-fixed MCF cells using ESR1 Antibody (OAEC00072, Red)
 

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