Raf-1 Antibody (Phospho-Ser259) (OAEC00006)

• Product Number: OAEC00006
• Name: Raf-1 Antibody (Phospho-Ser259) (OAEC00006)
• Molecular Weight: 73 kDa
• NCBI Gene Id: 5894
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: RAF proto-oncogene serine/threonine-protein kinase
• Alias Symbols: NS5, CRAF, Raf-1, c-Raf, CMD1NN
• Product Format: Liquid
• Reference: Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224. Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468. Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183.
• Reconstitution and Storage: Store at -20C/1 year
• Specificity: Raf-1 (phospho-Ser259) antibody detects endogenous levels of Raf-1 only when phosphorylated at serine 259.
• Application Info: WB: 1:500-1:1000, IHC: 1:50-1:100
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-S^P-T-P).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P04049
• Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• Protein Accession #: NP_002871.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation
• Gene Symbol: RAF1
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC
• Image 1: Human breast carcinoma
  
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf-1 (phospho-Ser259) antibody (OAEC00006
• Image 2: Raf-1 (Ab-259)
  
  Western blot analysis using Raf-1 (Ab-259) antibody (OAEC00356, Line 1, 2, and 3) and Raf-1 (phospho- Ser259) antibody (OAEC00006, Line 4, 5, and 6