| Product Number |
OAEB02481 |
| Product Page |
www.avivasysbio.com/foxc1-antibody-c-terminal-region-oaeb02481.html |
| Name |
FOXC1 Antibody - C-terminal region (OAEB02481) |
| Molecular Weight |
56.8 |
| NCBI Gene Id |
17300|2296 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
forkhead box C1 |
| Alias Symbols |
ARA;ASGD3;ch;congenital hydrocephalus;fkh-1;Fkh1;FKHL7;forkhead box C1 protein;forkhead box protein C1;forkhead, drosophila, homolog-like 7;forkhead/winged helix-like transcription factor 7;forkhead-related activator 3;forkhead-related protein FKHL7;forkhead-related transcription factor 3;FREA;FREAC3;FREAC-3;frkh;frkhda;IGDA;IHG1;IRID1;mesoderm/mesenchyme forkhead 1;Mf1;MF-1;Mf4;myeloid factor-delta;RIEG3;transcription factor FKH-1. |
| Peptide Sequence |
RTSGAFVYDCSKF |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for FOXC1 Antibody - C-terminal region (OAEB02481) |
| Additional Information |
Epitope Info: C Terminus
Domains: COG5025, Transcription factor of the Forkhead/HNF3 family [Transcription]; Fork head domain |
| Additional Information |
Reactivity Info: Tested: Human, Mouse; Expected: Human, Mouse |
| Application Info |
Peptide ELISA: Antibody detection limit dilution 1:32000.
Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining of some cells in the glomeruli and in paraffin embedded Human Spleen shows nucleolar staining. Recommended concentration, 3 ug/ml.
Western Blot: Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 56.8kDa according to NP_001444.1). Recommended concentration: 0.5-1.5ug/ml. |
| Immunogen |
FOXC1 |
| Uniprot ID |
Q12948 |
| Protein Name |
Forkhead box protein C1 |
| Protein Accession # |
NP_001444.2 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Foxc1|FOXC1 |
| Predicted Species Reactivity |
Human|Mouse|Rat |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Human: 100%; Mouse: 100%; Zebrafish: 100% |
| Image 1 | HEK293
 | HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with OAEB02481 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with OAEB02481 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. |
| | Image 2 | Human kidney
 | OAEB02481 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. |
|
|
