| Product Number |
OAEB02390 |
| Product Page |
www.avivasysbio.com/wnt3-antibody-oaeb02390.html |
| Name |
WNT3 Antibody (OAEB02390) |
| Molecular Weight |
39.6 |
| NCBI Gene Id |
22415|24882|7473 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
wingless-type MMTV integration site family, member 3|Wnt family member 3 |
| Alias Symbols |
Int;Int-4;INT4;proto-oncogene Int-4;proto-oncogene Int-4 homolog;proto-oncogene protein Wnt-3;proto-oncogene Wnt-3;TETAMS;wingless-related MMTV integration site 3;Wingless-type MMTV integration site 3, homolog;wingless-type MMTV integration site family, member 3;Wnt-;Wnt-3;WNT-3 proto-oncogene protein. |
| Peptide Sequence |
CGRGHNTRTEKRKEK |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Schmitt AM, Shi J, Wolf AM, Lu CC, King LA, Zou Y.; Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping.; Nature. 2006 Jan 5;439(7072):31-7.; Pubmed 16280981 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for WNT3 Antibody (OAEB02390) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:2000.
Western Blot: Approx 38kDa band observed in Human Brain lysates (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Recommended concentration, 3-5ug/ml. |
| Immunogen |
WNT3 |
| Uniprot ID |
P56703 |
| Protein Name |
Proto-oncogene Wnt-3 |
| Protein Accession # |
NP_110380.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Wnt3|WNT3 |
| Predicted Species Reactivity |
Dog|Human|Mouse|Rat |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | HEK293
 | OAEB02390 (1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| | Image 2 | Human Breast
 | EB7253 (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. |
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