Goat Anti-FOXO4 / MLLT7 Antibody (OAEB02081)

Data Sheet
 
Product Number OAEB02081
Product Page www.avivasysbio.com/goat-anti-foxo4-mllt7-antibody-oaeb02081.html
Name Goat Anti-FOXO4 / MLLT7 Antibody (OAEB02081)
Molecular Weight 53.7
NCBI Gene Id 4303
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name forkhead box O4
Alias Symbols AFX;AFX1;fork head domain transcription factor AFX1;forkhead box protein O4;MLLT7;myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7.
Peptide Sequence C-EAASQDRMPQDLD
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Paik JH, Kollipara R, Chu G, Ji H, Xiao Y, Ding Z, Miao L, Tothova Z, Horner JW, Carrasco DR, Jiang S, Gilliland DG, Chin L, Wong WH, Castrillon DH, DePinho RA.; FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis.; Cell. 2007 Jan 26;128(2):309-23.; Pubmed 17254969
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for Goat Anti-FOXO4 / MLLT7 Antibody (OAEB02081)
Additional Information Reactivity Info: Tested: Human; Expected: Human
Application Info Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot: Approx 60-65kDa band observed in lysates of cell line K562 (calculated MW of 53.7kDa according to NP_005929.2). Recommended concentration: 1-3ug/ml.
Immunogen FOXO4
Uniprot ID P98177
Protein Name Forkhead box protein O4
Protein Accession # NP_005929.2
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol FOXO4
Predicted Species Reactivity Human
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 92%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 76%; Pig: 92%; Rabbit: 100%; Rat: 90%
Image 1
K562
OAEB02081 (1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Image 2
Rabbit antibody
OAEB02081 (0.5ug/ml) as the capture rabbit antibody (2.5ug/ml).
 

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