Product Number |
OAEB00865 |
Product Page |
www.avivasysbio.com/goat-anti-pik3ca-p110alpha-antibody-oaeb00865.html |
Name |
Goat Anti-PIK3CA / P110alpha Antibody (OAEB00865) |
Molecular Weight |
124 |
NCBI Gene Id |
170911|18706|5290 |
Host |
Goat |
Clonality |
Polyclonal |
Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration |
0.5 mg/ml |
Gene Full Name |
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha|phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha |
Alias Symbols |
6330412C24Rik;caP;caPI3K;CLAPO;CLOVE;CWS5;MCAP;MCM;MCMTC;mutant phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha;p110;p110alpha;p110-alpha;phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha;phosphatidylinositol 3-kinase, catalytic, alpha polypeptide;phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform;phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;phosphoinositide 3-kinase alpha;phosphoinositide-3-kinase catalytic alpha polypeptide;phosphoinositide-3-kinase, catalytic, alpha polypeptide;PI3K;PI3Kalpha;PI3K-alpha;PI3-kinase p110 subunit alpha;PI3-kinase subunit alpha;ptdIns-3-kinase subunit alpha;ptdIns-3-kinase subunit p110-alpha;serine/threonine protein kinase PIK3CA. |
Peptide Sequence |
C-HQWLKDKNKGEIYD |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reference |
* Abubaker J, Bavi P, Al-Haqawi W, Jehan Z, Munkarah A, Uddin S, Al-Kuraya KS.; PIK3CA alterations in Middle Eastern ovarian cancers.; Mol Cancer. 2009 Jul 28;8:51.; Pubmed 19638206 * Lopez-Knowles E, Hernandez S, Malats N, Kogevinas M, Lloreta J, Carrato A, Tardon A, Serra C, Real FX.; PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors.; Cancer Res. 2006 Aug 1;66(15):7401-4.; Pubmed 16885334 |
Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for Goat Anti-PIK3CA / P110alpha Antibody (OAEB00865) |
Application Info |
Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1ug/ml). |
Immunogen |
PIK3CA |
Uniprot ID |
P42336 |
Protein Name |
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform |
Protein Accession # |
NP_006209.2 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Gene Symbol |
Pik3ca|PIK3CA |
Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
Application |
P-ELISA, IHC |
Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
Image 1 | Human Brain
| OAEB00865 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebellar Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Image 2 | Immunohistochemistry
| OAEB00865 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebellar Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Image 3 | Immunohistochemistry
| OAEB00865 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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