XYLT2 Antibody (OACD01059)

Data Sheet
 
Product Number OACD01059
Product Page www.avivasysbio.com/xylt2-antibody-oacd01059.html
Name XYLT2 Antibody (OACD01059)
Molecular Weight 97 kDa
Isotype IgG
NCBI Gene Id 217119
Host Rabbit
Clonality Polyclonal
Concentration 0.68 mg/ml
Gene Full Name xylosyltransferase II
Alias Symbols E030002B02Rik;peptide O-xylosyltransferase 2;XT2;XT-II;xylosyltransferase 2;Xylosyltransferase II;xylT-II.
Product Format Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description of Target Involved in the formation of heparan sulfate and chondroitin sulfate proteoglycans (By similarity). Probably catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes (By similarity).
Reconstitution and Storage 2°C to 8°C|-20°C
Datasheets/Manuals Printable datasheet for XYLT2 Antibody (OACD01059)
Specificity The antibody is a rabbit polyclonal antibody raised against XYLT2. It has been selected for its ability to recognize XYLT2 in immunohistochemical staining and western blotting.
Immunogen Range: Phe596 - Arg865
Formulation PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
Uniprot ID Q9EPL0
Protein Name Xylosyltransferase 2
Protein Accession # NP_665827.2
Purification Affinity Purified; Protein A Purified
Application Data Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
Optimal working dilutions must be determined by end user.
Nucleotide Accession # NM_145828
Preservative 0.02% NaN3
Gene Symbol Xylt2
Predicted Species Reactivity Human|Mouse|Rat
Application Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Image 1
Xylosyltransferase 2
Western Blot with Mouse Recombinant Xylosyltransferase 2
Image 2
WT and KO HeLa Cell Lysate
Verification of specificity by knock-out - Lane 1: Wild-type Hela cell lysate, Lane 2: XYLT2 knockout Hela cell lysate, Predicted MW: 97kd Observed MW: 95kd, Primary Ab: 1 ug/mL Xylt2 Antibody (OACD01059), Secondary Ab: 0.2 ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Image 3
Kidney tissue
DAB staining on formalin fixed paraffin-embedded Kidney tissue
 

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