SMARCC2 Antibody (OACA02428)

• Product Number: OACA02428
• Name: SMARCC2 Antibody (OACA02428)
• Protein Size (# AA): 1214 amino acids
• Molecular Weight: 133 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 6601
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 2
• Alias Symbols: BAF170;BRG1-associated factor 170;chromatin remodeling complex BAF170 subunit;CRACC2;CSS8;mammalian chromatin remodeling complex BRG1-associated factor 170;Rsc8;SWI/SNF complex 170 kDa subunit;SWI/SNF complex subunit SMARCC2;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2;SWI3-like protein.
• Product Format: Liquid
• Description of Target: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:11018012). Can stimulate the ATPase activity of the catalytic subunit of these complexes (PubMed:10078207). May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells (PubMed:12192000). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation (By similarity).
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human SWI/SNF complex subunit SMARCC2 protein (300-650AA)
• Formulation: Provided in PBS, pH 7.3, 50% Glycerol, 0.02% Sodium Azide
• Uniprot ID: Q8TAQ2
• Protein Name: SWI/SNF complex subunit SMARCC2
• Protein Accession #: NP_001123892.1
• Purification: Affinity Purified
• Application Data: WB:1:200-1000, IHC:1:20-1:200
• Nucleotide Accession #: NM_001130420.1
• Gene Symbol: SMARCC2
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HeLa
  
  Hela (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-SMARCC2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.
• Image 2: Human Ovarian Cancer
  
  Immunohistochemistry of paraffin-embedded human ovarian cancer using OACA02428 at dilution 1: 100
• Image 3: Human Rectal Cancer
  
  Immunohistochemistry of paraffin-embedded human rectal cancer using OACA02428 at dilution 1: 100
• Image 4: Human Tonsil
  
  Immunohistochemistry of paraffin-embedded human tonsil using OACA02428 at dilution 1: 100
• Image 5: Mouse Liver
  
  All lanes: SWI/SNF complex subunit SMARCC2 antibody at 2ug/ml + Mouse liver tissue secondary Goat polyclonal to rabbit at 1/10,000 dilution predicted band size: 133 kDa observed band size: 133 kDa