Product Number |
OAAH00061 |
Product Page |
www.avivasysbio.com/hist1h3a-antibody-oaah00061.html |
Name |
HIST1H3A Antibody (OAAH00061) |
Protein Size (# AA) |
136 amino acids |
Molecular Weight |
16 kDa |
NCBI Gene Id |
8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; |
Host |
Rabbit |
Clonality |
Polyclonal |
Source |
Rabbit pAb |
Gene Full Name |
Histone H3.1 |
Alias Symbols |
H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A |
Reference |
1. Albig, W., Doenecke, D., Hum. Genet. 101: 284-294, 1997. 2. Albig, W., Ebentheuer, J., Klobeck, G., Hum. Genet. 97: 486-491, 1996. 3. Yamagishi, Y., Honda, T., Tanno, Y., Science 330: 239-233, 2010.
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Description of Target |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.This gene is intronless and encodes a member of the histone H3 family. |
Reconstitution and Storage |
Store the product at -20C. Stable for one year from the date of shipment. |
Datasheets/Manuals |
Printable datasheet for anti-HIST1H3A (OAAH00061) antibody |
Specificity |
H3K36me2 polyclonal antibody detects endogenous levels of Histone H3 di- methylated at Lys36. |
Additional Information |
Localization: Nucleus |
Application Info |
WB: 1:1000-1:4000 IF: 1:100-1:500 DB: 1:500
|
Immunogen |
This Aviva H3K36me2 polyclonal antibody was produced by immunizing rabbits with synthetic Histone H3 peptide with Lys36 dimethylated and conjugated to KLH. |
Uniprot ID |
P68431 |
Protein Name |
Histone H3.1 |
Protein Accession # |
NP_003520.1; NP_003521.2; NP_003522.1; NP_003523.1; NP_003524.1; NP_003525.1; NP_003526.1; NP_003527.1; NP_003528.1 |
Nucleotide Accession # |
NM_003529.2; NM_003530.4; NM_003531.2; NM_003532.2; NM_003533.2; NM_003534.2; NM_003535.2; NM_003536.2; NM_003537.3 |
Gene Symbol |
HIST1H3A |
Application |
WB, IF, DB, CHIP |
Image 1 | H3K36me2 peptide
| This antibody was tested on a peptide-chip (peptides loaded shown in the left panel) by dot blot assay, which showed a specific reaction with H3K36me2 peptide (right panel). me: methylated; ac: acetylated; as: asymmetric
Primary antibody: 1:500 |
| Image 2 | HCT116
| Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
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| Image 3 | 293T
| IF analysis of H3K36me2 in 293T cells. Green: anti-H3K36me2; Blue: DAPI
Primary antibody:1:500
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| Image 4 | 293T nuclear extract
| Endogenous H3K36me2 detected in 293T nuclear extract
Primary antibody: 1:1000
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