LPAR1 Antibody (OAAF04794)

• Product Number: OAAF04794
• Name: LPAR1 Antibody (OAAF04794)
• Molecular Weight: 41 kDa
• NCBI Gene Id: 1902
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: lysophosphatidic acid receptor 1
• Alias Symbols: edg-2;EDG2;endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2;Gpcr26;LPA receptor 1;LPA1;LPA-1;lysophosphatidic acid receptor 1;lysophosphatidic acid receptor Edg-2;Mrec1.3;rec.1.3;ventricular zone gene 1;vzg-1;VZG1.
• Product Format: Liquid
• Reference: An S., Biochem. Biophys. Res. Commun. 231:619-622(1997).
  Moolenaar W.H., Curr. Opin. Cell Biol. 9:168-173(1997).
  Contos J.J.A., Mol. Pharmacol. 58:1188-1196(2000).
• Description of Target: Receptor for lysophosphatidic acid (LPA) (PubMed:9070858, PubMed:19306925, PubMed:25025571, PubMed:26091040). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels (PubMed:26091040). Signaling triggers an increase of cytoplasmic Ca(2+) levels (PubMed:19656035, PubMed:19733258, PubMed:26091040). Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate (PubMed:19306925). Signaling mediates activation of down-stream MAP kinases (By similarity). Contributes to the regulation of cell shape. Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction (PubMed:26091040). Promotes the activation of Rho and the formation of actin stress fibers (PubMed:26091040). Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1 (By similarity). Through its function as lysophosphatidic acid receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding (PubMed:18066075, PubMed:19656035, PubMed:19733258). Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14. Promotes cell proliferation in response to lysophosphatidic acid. Required for normal skeleton development. May play a role in osteoblast differentiation. Required for normal brain development. Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus. Plays a role in pain perception and in the initiation of neuropathic pain (By similarity).
• Reconstitution and Storage: -20°C
• Specificity: EDG2 antibody detects endogenous levels of total EDG2 protein.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from human EDG2.
• Dilution: The optimal working dilution should be determined by the end user.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: Q92633
• Protein Name: Lysophosphatidic acid receptor 1
• Protein Accession #: NP_001392.2
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Gene Symbol: LPAR1
• Predicted Species Reactivity: Human|Rat
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: human brain tissue
  
  Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EDG2 Antibody. The picture on the right is treated with the synthesized peptide.
• Image 2: MCF7 cells
  
  Immunofluorescence analysis of MCF7 cells, using EDG2 Antibody. The picture on the right is treated with the synthesized peptide.
• Image 3: Jurkat cells
  
  Western blot analysis of extracts from Jurkat cells, using EDG2 Antibody. The lane on the right is treated with the synthesized peptide.